Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Eisenwort, G; Jurkin, J; Yasmin, N; Bauer, T; Gesslbauer, B; Strobl, H.
Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-β1-dependent human epidermal Langerhans cells.
J Invest Dermatol. 2011; 131(10):2049-2057
Doi: 10.1038/jid.2011.164
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Strobl Herbert
- Co-Autor*innen der Med Uni Graz
- Bauer Thomas
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Langerin (CD207) expression is a hallmark of epidermal Langerhans cells (LCs); however, CD207(+) cells comprise several functional subsets. Murine studies showed that epidermal, but not dermal, CD207(+) cells require transforming growth factor-β 1 (TGF-β1) for development, whereas human data are lacking. Using gene profiling, we found that the surface molecule TROP2 (TACSTD2) is strongly and rapidly induced during TGF-β1-dependent LC commitment of human CD34(+) hematopoietic progenitor cells or monocytes. TROP2 is conserved between mouse and human, and shares substantial amino-acid identity with EpCAM, a marker for murine epidermal LCs. To our knowledge, neither TROP2 nor EpCAM expression has been analyzed in human dendritic cell (DC) subsets. We found that (i) all human epidermal LCs are TROP2(+)EpCAM(+); (ii) human dermis lacks CD207(+)EpCAM(-) or CD207(+)TROP2(-) DCs, i.e., equivalents of murine dermal CD207(+) DCs; and (iii) pulmonary CD207(+) cells are TROP2(-)EpCAM(-). Moreover, although EpCAM was broadly expressed by pulmonary and intestinal epithelial cells, as well as by bone marrow erythroid progenitor cells, these cells lacked TROP2. However, although TROP2 is expressed by human LCs as well as by human and murine keratinocytes, most murine LCs, except of a small subset, lacked TROP2. Therefore, TROP2 is a marker for human TGF-β1-dependent epidermal LCs.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, CD - metabolism
- Antigens, CD14 - biosynthesis
- Antigens, CD34 - biosynthesis
- Antigens, Neoplasm - metabolism
- Antigens, Surface - metabolism
- Cell Adhesion Molecules - metabolism
- Cell Movement -
- Cell Separation -
- Dendritic Cells - cytology
- Epidermis - metabolism
- Flow Cytometry -
- Hematopoietic Stem Cells - cytology
- Humans -
- Langerhans Cells - cytology
- Lectins, C-Type - metabolism
- Mannose-Binding Lectins - metabolism
- Mice -
- Monocytes - cytology
- Stem Cells - cytology
- Transforming Growth Factor beta1 - metabolism