Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Strobl, H; Takimoto, M; Majdic, O; Höcker, P; Knapp, W.
Antigenic analysis of human haemopoietic progenitor cells expressing the growth factor receptor c-kit.
Br J Haematol. 1992; 82(2):287-294
Doi: 10.1111/j.1365-2141.1992.tb06420.x
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- Führende Autor*innen der Med Uni Graz
- Strobl Herbert
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- Abstract:
- The cell surface molecule encoded by the protooncogene c-kit has recently been identified as the receptor for a growth factor variously termed stem cell factor (SCF), mast cell growth factor or steel factor. Using the c-kit antibody 17F11 we analysed, in triple staining experiments, the surface molecule profile and scatter characteristics of c-kit+CD34+ human haemopoietic progenitor cells. In 10 normal bone marrow samples we found 19-51% of CD34+ bone marrow progenitor cells to coexpress c-kit. These c-kit+CD34+ bone marrow cells turned out to represent a phenotypically heterogeneous population. A considerable proportion coexpressed CD33 (52 +/- 23%), and/or CD71 (62 +/- 26) antigens, marker molecules previously shown to be expressed by committed in vitro colony forming cells but not by their precursors. In line with a relatively differentiated phenotype c-kit+CD34+ cells also gave rise to on average higher forward and right-angle light scattering signals. The proportions of CD38 and/or HLA-D expressing cells were similar in the c-kit+ and in the c-kit- subsets of CD34+ progenitor cells. Coexpression of CD19 was found to be less frequent in the c-kit+ (4 +/- 5%) as compared to the c-kit- (17 +/- 14%) fraction of CD34+ cells. CD7+ CD34+ bone marrow cells were hardly detectable and their numbers too low to allow further subdivision in c-kit+ and c-kit- subsets.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD - analysis
- Antigens, CD34 -
- Antigens, Surface - analysis
- Bone Marrow - immunology
- HLA-D Antigens - analysis
- Hematopoietic Stem Cells - immunology
- Humans -
- Proto-Oncogene Proteins - analysis
- Proto-Oncogene Proteins c-kit -
- Receptors, Colony-Stimulating Factor - analysis