Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bello-Fernández, C; Matyash, M; Strobl, H; Scheinecker, C; Knapp, W.
Analysis of myeloid-associated genes in human hematopoietic progenitor cells.
Exp Hematol. 1997; 25(11):1158-1166
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Strobl Herbert
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- Abstract:
- The distribution of myeloid lineage-associated cytokine receptors and lysosomal proteins was analyzed in human CD34+ cord blood cell (CB) subsets at different stages of myeloid commitment by reverse-transcriptase polymerase chain reaction (RT-PCR). The highly specific granulomonocyte-associated lysosomal proteins myeloperoxidase (MPO) and lysozyme (LZ), as well as the transcription factor PU.1, were already detectable in the most immature CD34+Thy-1+ subset. Messenger RNA (mRNA) levels for the granulocyte-colony stimulating factor (G-CSF) receptor, granulocyte-macrophage (GM)-CSF receptor alpha subunit and tumor necrosis factor (TNF) receptors I (p55) and II (p75) were also detected in this subset in addition to c-kit and flt-3, receptors known to be expressed on progenitor cells. By contrast, the monocyte-macrophage colony stimulating factor (M-CSF) receptor was largely absent at this stage and in the CD34+Thy-1-CD45RA- subsets. The M-CSF receptor was first detectable in the myeloid-committed CD34+Thy-l-CD45RA+ subset. All other molecules studied were found to be expressed at this stage of differentiation. Different cocktails of the identified ligands were added to sorted CD34+Thy-1+ single cells. Low proliferative capacity was observed after 1 week in culture in the presence of stem cell factor (SCF) + Flt-3 ligand (FL) + G-CSF. Addition of GM-CSF to this basic cocktail consistently increased the clonogenic capacity of single CD34+Thy-1+ cells, and this effect was further enhanced (up to 72.3 +/- 4.3% on day 7) by the inclusion of TNF-alpha. In conclusion, the presence of myeloid-associated growth factor receptor transcripts in CD34+ CB subsets does not discriminate the various stages of differentiation, with the exception of the M-CSF receptor. In addition, we show that TNF-alpha is a potent costimulatory factor of the very immature CD34+Thy-1+ CB subset.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD - analysis
- Antigens, CD34 - analysis
- Fetal Blood - cytology
- Flow Cytometry -
- Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - immunology Hematopoietic Stem Cells - physiology
- Humans -
- Infant, Newborn -
- Lysosomes - metabolism
- Muramidase - biosynthesis
- Peroxidase - biosynthesis
- Polymerase Chain Reaction -
- Proto-Oncogene Proteins - biosynthesis
- RNA, Messenger - biosynthesis
- Receptors, Cytokine - biosynthesis
- Th1 Cells - cytology Th1 Cells - immunology Th1 Cells - physiology
- Trans-Activators - biosynthesis
- Transcription, Genetic -
- Find related publications in this database (Keywords)
- CD34(+) CB subsets
- RT-PCR
- myeloid cytokine receptors
- lysosomal proteins