Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kapiotis, S; Quehenberger, P; Sengoelge, G; Pärtan, C; Eher, R; Strobl, H; Bevec, D; Zapolska, D; Schwarzinger, I; Speiser, W.
Modulation of pyrogen-induced upregulation of endothelial cell adhesion molecules (CAMs) by interleukin-4: transcriptional mechanisms and CAM-shedding.
Circ Shock. 1994; 43(1):18-25
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Strobl Herbert
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- Abstract:
- The pyrogens interleukin 1 (IL-1), tumor necrosis factor (TNF), and bacterial lipopolysaccharides (LPS) are known to increase endothelial cell (EC) adhesiveness for leukocytes by stimulating surface expression of various adhesion molecules. IL-4, a product of activated T-cells, was shown to affect pyrogen-mediated regulation of EC adhesion molecule surface expression. In the present study, we investigated the effect of IL-4 on pyrogen-induced upregulation of the cell adhesion molecules (CAMs) ICAM-1 (intercellular cell adhesion molecule-1), ELAM-1 (endothelial leucocyte adhesion molecule-1), and VCAM-1 (vascular cell adhesion molecule-1) in cultured human umbilical vein EC (HUVEC). Surface expression of adhesion molecules was quantified by flow cytometry, HUVEC mRNA content was estimated by Northern blot analysis, and ICAM-1 antigen in conditioned media was measured by ELISA. Incubation of HUVEC with IL-1 (100 U/ml), TNF (500 U/ml), and LPS (10 micrograms/ml) caused significant increase in ICAM-1, ELAM-1, and VCAM-1 surface expression; IL-1 caused about an eightfold increase in ICAM-1 expression, about a 13-fold increase in ELAM-1 surface expression, and about a fourfold increase in VCAM-1 expression. Coincubation of pyrogens with IL-4 (500 U/ml) differentially influenced their proadhesive effects on the HUVEC surface. In the presence of IL-4, IL-1-induced ICAM-1 upregulation was reduced, ELAM-1 upregulation was not significantly influenced by IL-4, and induction of VCAM-1 was enhanced by IL-4.(ABSTRACT TRUNCATED AT 250 WORDS)
- Find related publications in this database (using NLM MeSH Indexing)
- Base Sequence -
- Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism
- Cells, Cultured -
- E-Selectin -
- Endothelium, Vascular - metabolism
- Escherichia coli -
- Humans -
- Intercellular Adhesion Molecule-1 - genetics Intercellular Adhesion Molecule-1 - metabolism
- Interleukin-1 - pharmacology
- Interleukin-4 - pharmacology
- Lipopolysaccharides - pharmacology
- Molecular Sequence Data -
- Pyrogens - pharmacology
- RNA, Messenger - metabolism
- Transcription, Genetic -
- Tumor Necrosis Factor-alpha - pharmacology
- Umbilical Veins -
- Vascular Cell Adhesion Molecule-1 -
- Find related publications in this database (Keywords)
- ENDOTHELIUM
- CYTOKINES
- ADHESION MOLECULES
- TRANSCRIPTION