Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Strobl, H; Knapp, W.
Myeloid cell-associated lysosomal proteins as flow cytometry markers for leukocyte lineage classification.
J Biol Regul Homeost Agents. 2005; 18(3-4):335-339
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Strobl Herbert
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- Abstract:
- Lysosomal proteins including myeloperoxidase (MPO), lysozyme (LZ), CD68 and lactoferrin (LF), represent classical immunohistology marker molecules. Additionally, flow cytometry can be used to detect and quantify their expression at the single cell level in phenotypically defined leukocyte subsets. Recent results demonstrated that expression densities of these intracellular proteins vary among myeloid cell subsets, thus enabling insights into novel subset biology and development. Additionally, whole blood staining protocols allow detection of lysosomal proteins in infrequent leukocyte subsets such as circulating CD34+ hematopoietic progenitors and dendritic cells (DC). Thus, information on leukocyte subset distribution and aberrant phenotypes might be gained for diagnositic purposes. Finally, FACS detection of MPO and LZ proved to be of high value for the lineage diagnosis of acute leukemias.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD - analysis
- Antigens, Differentiation, Myelomonocytic - analysis
- Biological Markers -
- Cell Lineage -
- Flow Cytometry -
- Humans -
- Leukemia, Myeloid, Acute - diagnosis
- Leukocytes - classification
- Muramidase - analysis
- Myelopoiesis -
- Neoplasm, Residual -
- Peroxidase - analysis
- Proteins - analysis
- Find related publications in this database (Keywords)
- leukemia diagnosis
- dendritic cells
- hematopoiesis
- CD34
- myeloperoxidase
- lysozyme
- CD68