Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Pavlova, BG; Mühlberger, HH; Strobl, H; Grill, R; Haslberger, A; Varga, F; Auer, H; Heinz, R; Salamon, J; Stacher, A.
B lymphocytes with latent EBV infection appearing in long-term bone marrow cultures (HLTBMCs) from haematological patients induce lysis of stromal microenvironment.
Br J Haematol. 1995; 89(4):704-711
Doi: 10.1111/j.1365-2141.1995.tb08405.x
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- Co-Autor*innen der Med Uni Graz
- Strobl Herbert
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- Abstract:
- Human long-term bone marrow cultures (HLTBMCs) are a valuable in vitro model for studying the role of the haemopoietic microenvironment. Here we report the spontaneous appearance of EBV-positive B cells in 6/40 HLTBMCs from patients with various haematological diseases after 3-5 months of culture. After subcultivation of these cells, a novel type of cell line could be characterized, which displayed surface markers and morphological features typical for EBV transformed B-cell lines. As the deproteinized and ultrafiltrated culture supernatants of these cell lines were found to contain an agent with stroma toxic properties, they were termed SSB lines (stroma-toxic-agent-secreting B-cell lines). This agent also exhibited a colony-inhibitory activity on in vitro myelopoiesis and erythropoiesis. These properties are typical for the two polyamines spermine and spermidine which were detected at elevated levels in the culture supernatants of SSB lines. The hypothesis that latent presence of EBV in bone marrow may induce an increased synthesis of spermine and spermidine, which are known to be associated with malignant haematological diseases and bone marrow aplasia, is discussed.
- Find related publications in this database (using NLM MeSH Indexing)
- B-Lymphocytes - pathology B-Lymphocytes - virology
- Bone Marrow - pathology
- Cell Line, Transformed -
- Cell Transformation, Viral -
- Erythroid Precursor Cells - drug effects Erythroid Precursor Cells - pathology
- Hematologic Diseases - metabolism Hematologic Diseases - pathology Hematologic Diseases - virology
- Herpesvirus 4, Human -
- Humans -
- Receptors, Complement 3d -
- Receptors, Virus - analysis
- Spermidine - biosynthesis Spermidine - pharmacology
- Spermine - biosynthesis Spermine - pharmacology
- Tumor Cells, Cultured -
- Virus Latency -
- Find related publications in this database (Keywords)
- HLTBMCS
- EBV-POSITIVE B LYMPHOCYTES
- STROMA CELL LYSIS