Medizinische Universität Graz - Research portal
Selected Publication:
Schnedl, WJ; Reisinger, EC; Katzensteiner, S; Lipp, RW; Schreiber, F; Hopmeier, P; Krejs, GJ.
Haemoglobin O Padova and falsely low haemoglobin A1c in a patient with type I diabetes.
J Clin Pathol. 1997; 50(5):434-435
Doi: 10.1136/jcp.50.5.434
(- Case Report)
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Leading authors Med Uni Graz
- Schnedl Wolfgang
- Co-authors Med Uni Graz
- Krejs Günter Josef
- Lipp Rainer
- Schreiber Florian
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Glycated haemoglobin (HbA1c) measured by high performance liquid chromatography (HPLC) in a 20 year old female with insulin dependent diabetes mellitus was consistently within the normal range although her daily blood glucose values were > 11.1 mmol/l. HbA1c measured by immunoagglutination and fructosamine was elevated and correlated with the patient's blood glucose values. The HPLC chromatogram showed an additional peak at HbA0. Electrophoresis of haemoglobin on citrate agar gel revealed an abnormal haemoglobin anodal of HbS. Cellulose acetate electrophoresis and isoelectric focusing demonstrated an additional haemoglobin migrating close to HbA2. Amino acid analysis and DNA sequencing revealed an alpha 30 (B11) Glu-->Lys replacement, that is, haemoglobin O Padova. Investigations of two family members without diabetes revealed the same rare haemoglobin variant. This case showed that this silent haemoglobin mutation caused an additional peak and falsely low HbA1c values when measured by HPLC, the gold standard for this evaluation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Biomarkers - blood
- Chromatography, High Pressure Liquid -
- Diabetes Mellitus, Type 1 - blood
- False Negative Reactions -
- Female -
- Glycated Hemoglobin A - analysis
- Hemoglobins, Abnormal - analysis
- Humans -
- Find related publications in this database (Keywords)
- High Performance Liquid Chromatography
- Hemoglobin Variants
- Insulin Dependent Diabetes Mellitus