The influence of cyclic AMP on gastrin release from the canine antrum was investigated by a direct in vivo method. After removal of spleen, gastric corpus, pancreas and intestine an antral pouch was consecutively perfused with dibutyryl cyclic AMP, papaverin and bethanechol-HCl using a branch of the left gastroepiploic artery. A relevant rise of gastrin in portal blood was only observed during perfusion of bethanechol chloride. Thus cyclic AMP does not release gastrin from the canine antrum.
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