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SHR Neuro Cancer Cardio Lipid Metab Microb
Alge-Priglinger, CS; André, S; Schoeffl, H; Kampik, A; Strauss, RW; Kernt, M; Gabius, HJ; Priglinger, SG.
Negative regulation of RPE cell attachment by carbohydrate-dependent cell surface binding of galectin-3 and inhibition of the ERK-MAPK pathway.
Biochimie. 2011; 93(3):477-488
Doi: 10.1016/j.biochi.2010.10.021
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Strauß Rupert
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- Abstract:
- Adhesion and spreading of retinal pigment epithelial (RPE) cells on fibronectin-rich extracellular matrices is a crucial event in the pathogenesis of proliferative vitreoretinopathy (PVR). In the present study we explored the capacity of galectin-3, a β-galactoside-binding endogenous lectin, to inhibit early PVR-associated cellular events from a therapeutic perspective. We assessed the relative expression levels of galectin-3 in native RPE and dedifferentiated, cultured RPE. Galectin-3 was constitutively expressed under in vivo and in vitro conditions and was abundant in cultured cells. Treatment of human RPE cells with soluble galectin-3 disclosed no toxicity within control limits up to 250 μg/ml. When added to the medium, galectin-3 dose-dependently inhibited attachment and spreading of the cells on fibronectin by more than 75%. When coated on the plastic surface, galectin-3 alone impaired attachment and spreading of RPE cells, and reduced attachment but not spreading on fibronectin. Galectin-3 bound to the cell surface, and, as determined by the use of the competing sugar β-lactose, galectin-3-mediated effects were dependent on carbohydrate binding. To ascertain the role of the ability of galectin-3 to form pentamers, we proteolytically removed the N-terminal, cross-linking section. The remaining C-terminal carbohydrate-binding domain alone failed to bind to cells and was functionally inactive. These results emphasize the relevance of both properties, i.e., glycan-binding and cross-linking of glycan moieties, for the inhibitory activity of galectin-3. Incubation of mobilized RPE cells with galectin-3 significantly disturbed microfilament assembly and, in correlation with decreased attachment, inhibited ERK phosphorylation. Therefore, galectin-3, acting as a cross-linking lectin on the cell surface, negatively regulates attachment and spreading of RPE cells in vitro. This effect, at least in part, is attributed to an inhibition of the ERK-MAPK pathway, which prevents cytoskeletal rearrangements needed for RPE cell attachment and spreading. Further investigation at this pathway may disclose a promising nouveau perspective for treatment and prophylaxis of early PVR.
- Find related publications in this database (using NLM MeSH Indexing)
- Actin Cytoskeleton - metabolism
- Aged -
- Binding, Competitive -
- Carbohydrate Metabolism -
- Cell Adhesion -
- Cell Shape -
- Cell Survival -
- Extracellular Signal-Regulated MAP Kinases - metabolism
- Female -
- Galectin 1 - metabolism
- Galectin 3 - biosynthesis
- Gene Expression Regulation -
- Humans -
- MAP Kinase Signaling System -
- Male -
- Middle Aged -
- Mitogen-Activated Protein Kinase 1 - metabolism
- Mitogen-Activated Protein Kinase 3 - metabolism
- Mitogen-Activated Protein Kinases - metabolism
- Phosphorylation -
- Protein Binding -
- Retinal Pigment Epithelium - cytology
- Find related publications in this database (Keywords)
- Attachment
- Cytoskeleton
- Galectin-3
- MAPK
- Proliferative
- Vitreoretinopathy
- Retinal pigment epithelium