Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Steyrer, E; Kostner, GM.
Interaction of lipoprotein Lpa with the B/E-receptor: a study using isolated bovine adrenal cortex and human fibroblast receptors.
J LIPID RES 1990 31: 1247-1253.
Doi: 10.1016/S0022-2275(20)42633-1
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- Führende Autor*innen der Med Uni Graz
- Kostner Gerhard
- Steyrer Ernst
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- Abstract:
- The ability of different lipoprotein Lp[a] preparations to compete with LDL-binding to the B/E-receptor was investigated by ligand blot and filter assays. Lp[a] was purified from donors with various genetic polymorphic forms by affinity chromatography using lysine-Sepharose or specific immunoadsorbers. These preparations were free of "LDL-like" material. Part of Lp[a] was reduced and freed from specific apo[a] antigen yielding "Lpa-." 125I-labeled low density lipoproteins (LDL) were incubated with B/E-receptor preparations from bovine adrenal cortex or from human skin fibroblasts, and the competition with unlabeled LDL, Lp[a], Lpa-, apo[a], and apoE-free HDL was studied by a ligand blot or filter assay technique. The following results were obtained. 1) LDL and Lpa- were equally potent in displacing 125I-labeled from B/E-receptor in the ligand blot and the filter assay. Lpa + ( = Lp[a]) also displaced LDL but to a much lesser degree: 50% displacement was observed with LDL and Lpa- at a 1-fold excess, whereas a 7.5-fold excess was required of Lpa +. 2) Apo[a], as well as apoE-free HDL, did not compete with LDL binding. 3) Competition experiments using B/E-receptors from bovine adrenal cortex or from human skin fibroblasts were comparable. 4) There was no difference in the behavior of Lp[a] isolated from the two affinity chromatography methods. 5) Lp[a] of different genetic variants behaved virtually identically. The results are discussed from the point of view of the in vivo metabolism of Lp[a].
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenal Cortex - metabolism
- Animals - metabolism
- Binding, Competitive - metabolism
- Blotting, Western - metabolism
- Cattle - metabolism
- Chromatography, Affinity - metabolism
- Fibroblasts - metabolism
- Humans - metabolism
- Ligands - metabolism
- Lipoprotein(a) - metabolism
- Lipoproteins - isolation and purification
- Lipoproteins, HDL - metabolism
- Lipoproteins, LDL - metabolism
- Receptors, LDL - metabolism