Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kuntner, C; Wanek, T; Hoffer, M; Dangl, D; Hornof, M; Kvaternik, H; Langer, O.
Radiosynthesis and assessment of ocular pharmacokinetics of (124)I-labeled chitosan in rabbits using small-animal PET.
Mol Imaging Biol. 2011; 13(2):222-226
Doi: 10.1007/s11307-010-0352-7
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Kvaternik Herbert
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- Abstract:
- The polysaccharide chitosan is a unique material for the design of ocular drug-delivery vehicles. The aim of this study was to radiolabel chitosan with iodine-124 (I-124) for measurement of ocular pharmacokinetics in rabbits using small-animal positron emission tomography (PET). Crl:CHBB (HM) rabbits received one drop (35 A mu L) of either a formulation containing chitosan (0.5%, w/v) spiked with I-124-labeled chitosan ([I-124]chitosan) (n = 4) or a control solution of sodium [I-124]iodide in buffer (n = 2) in the conjunctival sac of the right eye. Radioactivity distribution in the head region was measured at five different time points between 0 and 22 h after topical application. Regions of interest were manually defined in the reconstructed PET images, and activity concentration was quantified as percent applied dose (AD) per cubic centimeter tissue. Clear differences were observed in the ocular pharmacokinetics of the two formulations. At 3 h after application, ocular activity uptake was 0.5 +/- 0.1%AD/cc for sodium [I-124]iodide, compared to 4.7 +/- 5.3%AD/cc for the [I-124]chitosan formulation. We were able to show that ocular pharmacokinetics of I-124-labeled ophthalmic formulations can be measured with small-animal PET and that [I-124]chitosan had approximately a 2-fold increased ocular retention through the study period compared to sodium [I-124]iodide.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Area Under Curve -
- Chitosan - chemical synthesis Chitosan - pharmacokinetics
- Eye - radionuclide imaging
- Female -
- Iodine Radioisotopes -
- Isotope Labeling -
- Positron-Emission Tomography -
- Rabbits -
- Time Factors -
- Find related publications in this database (Keywords)
- Chitosan
- Iodine-124
- Small-animal PET
- Ocular pharmacokinetics