Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Ruckenstuhl, C; Eidenberger, A; Lang, S; Turnowsky, F.
Single amino acid exchanges in FAD-binding domains of squalene epoxidase of Saccharomyces cerevisiae lead to either loss of functionality or terbinafine sensitivity.
Biochem Soc Trans. 2005; 33(Pt 5):1197-1201
Doi: 10.1042/BST20051197
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- Leading authors Med Uni Graz
- Ruckenstuhl Rudolf
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- Abstract:
- Squalene epoxidase (Erg1p) is an essential enzyme in the ergosterol biosynthesis pathway in yeast. For its enzymatic activity, Erg1p requires molecular oxygen, NAD(P)H and FAD. Amino acid analysis and sequence alignment with other squalene epoxidases revealed two highly conserved FAD-binding domains, FAD I and FAD II. By random PCR mutagenesis of the ERG1 gene, one erg1 allele was isolated that carries a mutation leading to a single amino acid exchange in the FAD I domain close to the N-terminus of Erg1p. This erg1 allele codes for functional squalene epoxidase and renders yeast cells hypersensitive to terbinafine. Amino acid exchanges of other conserved residues in the FAD I and FAD II regions either led to non-functional squalene epoxidase or to the formation of squalene epoxidase with wild-type properties. These results describe the importance of specific amino acids for enzymatic activity in the yeast squalene epoxidase Erg1p.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Binding Sites -
- Conserved Sequence -
- Flavin-Adenine Dinucleotide - metabolism
- Humans -
- Molecular Sequence Data -
- NAD - metabolism
- NADP - metabolism
- Phylogeny -
- Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism
- Sequence Alignment -
- Sequence Homology, Amino Acid -
- Squalene Monooxygenase - chemistry Squalene Monooxygenase - metabolism
- Find related publications in this database (Keywords)
- allylamine
- ergosterol synthesis
- FAD-binding site
- squalene epoxidase
- terbinafine sensitivity
- yeast