Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schauer, A; Knauer, H; Ruckenstuhl, C; Fussi, H; Durchschlag, M; Potocnik, U; Fröhlich, KU.
Vacuolar functions determine the mode of cell death.
Biochim Biophys Acta. 2009; 1793(3):540-545
Doi: 10.1016/j.bbamcr.2008.11.006
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- Co-authors Med Uni Graz
- Ruckenstuhl Rudolf
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- Abstract:
- The yeast vacuole plays a crucial role in cell homeostasis including pH regulation and degradation of proteins and organelles. Class C VPS genes code for proteins essential for vacuolar and endosomal vesicle fusion, their deletion results in the absence of a detectable vacuole. We found that single gene deletions of class C VPS genes result in a drastically enhanced sensitivity to treatment with acetic acid whereas sensitivity towards H2O2 remains largely unaffected. Interestingly acetic acid treatment known as an established inducer of yeast apoptosis leads to necrosis in class C VPS deletion strains. Their intracellular pH drops from 6.7 to 5.5 after acetic acid treatment, while in wild type the pH drops to just 6.3. When the intracellular pH in wild type is lowered below pH 5.5 using a higher concentration of acetic acid, the survival rate is similarly low as in the class C VPS mutants, however, the death phenotype is predominantly apoptotic. Hence, the vacuole not only prevents acetic acid induced cell death by buffering the cytosolic pH, but it also has a proapoptotic function.
- Find related publications in this database (using NLM MeSH Indexing)
- Apoptosis -
- Hydrogen-Ion Concentration -
- Necrosis -
- Saccharomyces cerevisiae - metabolism
- Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism
- Vacuoles - metabolism
- Vesicular Transport Proteins - genetics Vesicular Transport Proteins - metabolism
- Find related publications in this database (Keywords)
- Vacuole
- Necrosis
- Apoptosis
- Acetic acid
- pH regulation