Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Beck, V; Davey, MG; Mayer, S; Froyen, G; Deckx, S; Klaritsch, P; Roubliova, XI; Petersen, SG; Deprest, JA.
A longer tracheal occlusion period results in increased lung growth in the nitrofen rat model.
Prenat Diagn. 2012; 32(1):39-44
Doi: 10.1002/pd.2881
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Klaritsch Philipp
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Objective Prenatal tracheal occlusion (TO) promotes lung growth and is applied clinically in fetuses with severe congenital diaphragmatic hernia. Limited data are available regarding the effect of duration of TO on lung development. Our objective was to evaluate the effects of long (2 and 2.5 days) versus short (1 day) TO on lung development in rats with nitrofen-induced diaphragmatic hernia. Method Nitrofen was administered on embryonic day (ED) 9 and fetal TO performed either on ED 18.5, 19 or 20 (term = 22 days). Sham operated and untouched littermates served as controls. On ED21, lungs were harvested and only fetuses with a left-sided diaphragmatic defect were included in further analyses. Results Lung body-weight ratio incrementally increased with the duration of TO. Increased proliferation following long TO was confirmed by immunohistochemistry and qRT-PCR for the proliferation marker Ki-67. Irrespective of duration, TO induced more complex airway architecture. Medial wall thickness of pulmonary arteries was thinner after long rather than short TO. Conclusion In the nitrofen rat model of congenital diaphragmatic hernia, a longer period of TO leads to enhanced lung growth and less muscularized pulmonary arteries. (C) 2011 John Wiley & Sons, Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Proliferation - drug effects
- Disease Models, Animal -
- Female -
- Fetal Diseases - surgery
- Fetus - abnormalities Fetus - surgery
- Gestational Age -
- Herbicides - toxicity
- Hernia, Diaphragmatic - chemically induced Hernia, Diaphragmatic - congenital Hernia, Diaphragmatic - pathology Hernia, Diaphragmatic - surgery
- Lung - drug effects Lung - embryology Lung - pathology
- Organ Size -
- Phenyl Ethers - toxicity
- Pregnancy -
- Rats -
- Rats, Wistar -
- Time Factors -
- Trachea - surgery
- Find related publications in this database (Keywords)
- congenital diaphragmatic hernia
- lung hypoplasia
- tracheal occlusion
- fetal surgery
- nitrofen rat