Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Pfragner, R; Höfler, H; Behmel, A; Ingolic, E; Walser, V.
Establishment and characterization of continuous cell line MTC-SK derived from a human medullary thyroid carcinoma.
CANCER RES. 1990; 50(13): 4160-4166.
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- Führende Autor*innen der Med Uni Graz
- Pfragner Roswitha
- Co-Autor*innen der Med Uni Graz
- Siegl Veronika
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- Abstract:
- Tumor cells of a human medullary thyroid carcinoma were isolated and propagated in tissue culture. Several cell lines with different morphology developed from the primary culture, among others a fibroblast-like growing cell line (MTC-F) and a cell line growing as a suspension of single cells and spherical cell clusters (MTC-SK). The MTC-SK cell line was serially propagated for 90 passages, over 3 years. When examined at different times throughout the in vitro period, MTC-SK exhibited properties characteristic of medullary thyroid carcinomas: the cells maintained their epithelioid morphology; endocrine granules were demonstrated in the cytoplasm by electron microscopy; in situ hybridization confirmed the production of calcitonin- and bombesin-mRNA (gastrin releasing peptide); the cells revealed positive immunoreactivity with antibodies to calcitonin, calcitonin gene-related peptide, and bombesin. The in vitro properties of the MTC-SK cells corresponded to the results obtained from the tissue of origin. Cytogenetic studies of the MTC-F cell line revealed a supernumerary metacentric chromosome (20?). In the MTC-SK cell line the predominant findings were terminal chromosomal rearrangements most frequently concerning chromosome 11p, i.e., the locus of the calcitonin and calcitonin gene-related peptide genes and the H-ras oncogene, and a characteristic instability of the centromeric region of chromosome 16 and somatic pairing of the homologous chromosomes 16.
- Find related publications in this database (using NLM MeSH Indexing)
- Calcitonin - analysis
- Calcitonin Gene-Related Peptide - analysis
- Carcinoma - analysis
- Chromosome Aberrations - analysis
- Female - analysis
- Gene Rearrangement - analysis
- Humans - analysis
- Karyotyping - analysis
- Middle Aged - analysis
- Somatostatin - analysis
- Thyroid Neoplasms - analysis
- Tumor Cells, Cultured - pathology