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SHR Neuro Cancer Cardio Lipid Metab Microb
Donnerer, J; Liebmann, I.
A fluorescence-immunohistochemical study on phosphorylation of ERK1/2, p38, and STAT3 in rat dorsal root ganglia following noxious stimulation of hind paw sensory neurons.
Tissue Cell. 2011; 43(3): 178-189.
Doi: 10.1016/j.tice.2011.02.002
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Donnerer Josef
- Co-authors Med Uni Graz
- Liebmann Ingrid
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- Abstract:
- A fluorescence-immunohistochemical investigation was performed in lumbar dorsal root ganglia (DRGs) neurons of the rat with regard to ERK1/2-, p38- and STAT3-phosphorylation in response to nociceptor activation in the rat. The stimuli applied were perineural capsaicin treatment of the sciatic nerve, mustard oil application to the hind paw and heat or cold stimulation of the hind paw. The time points of investigations were 15 min/30 min after perineural capsaicin, 30 min/2 h/4 h for mustard oil, 10 min/4 h for cold and 30 min/2 h/8 h for the heat stimulus. All four stimuli lead to a time-dependent, significant 2-3 fold increase in the number of small and medium size DRG cells displaying cytoplasmic staining for p-ERK1/2, but to no activation of satellite cells. Phosphorylated p38 immunoreactivity was increased in the cytoplasma of DRG cells at 2 h after the mustard oil treatment of the hind paw and 30 min after the perineural capsaicin application to the sciatic nerve axons, but not following heat or cold stimuli to the hind paws. Phospho-STAT3 staining was characteristically observed as nuclear and cytoplasmic staining. It was found increased after the perineural capsaicin application to the sciatic nerve axons, however, no marked increase was found with the other 3 noxious stimuli. The present results show that sensory neurons respond with a selective long-lasting increase in p-ERK1/2 in small and medium-size DRG cells, when their axons or axon terminals are stimulated by capsaicin, mustard oil, noxious heat or noxious cold.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Capsaicin - pharmacology
- Cold Temperature -
- Ganglia, Spinal - metabolism
- Hindlimb -
- Hot Temperature -
- Male -
- Mitogen-Activated Protein Kinase 1 - metabolism
- Mitogen-Activated Protein Kinase 3 - metabolism
- Mustard Plant -
- Neurons, Afferent - metabolism
- Nociceptors - drug effects Nociceptors - physiology
- Phosphorylation -
- Plant Oils - pharmacology
- Rats -
- Rats, Sprague-Dawley -
- STAT3 Transcription Factor - metabolism
- Sensory Receptor Cells - drug effects
- Signal Transduction - drug effects Signal Transduction - physiology
- TRPC Cation Channels - physiology
- p38 Mitogen-Activated Protein Kinases - metabolism
- Find related publications in this database (Keywords)
- Intracellular signalling
- ERK1/2
- p38
- STAT3
- Phosphorylation
- TRP channels
- Immunohistochemistry