Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Welz, W; Sattler, W; Leis, HJ; Malle, E.
Rapid analysis of non-esterified fatty acids as methyl esters from different biological specimens by gas chromatography after one-step esterification.
J Chromatogr. 1990; 526(2):319-329
Doi: 10.1016/S0378-4347(00)82516-5
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- Führende Autor*innen der Med Uni Graz
- Malle Ernst
- Co-Autor*innen der Med Uni Graz
- Leis Hans-Joerg
- Sattler Wolfgang
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- Abstract:
- A rapid gas chromatographic method for the determination of medium-chain and long-chain free fatty acids (C14:0 to C24:0 fatty acids) from different biological specimens is presented. After a rapid one-step transesterification method in methanol-acetyl chloride (50:1, v/v), fatty acid methyl esters were extracted into n-hexane and analysed on a 15-m Durabond-Wax column within a 12-min chromatographic run. The detection limit is 500 pg per injection.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Autoanalysis -
- Chromatography, Gas -
- Esterification -
- Fatty Acids, Nonesterified - analysis
- Humans - analysis
- Methylation - analysis
- Microsomes, Liver - analysis
- Rats - analysis
- Solvents - analysis