Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lipovsek, S; Letofsky-Papst, I; Hofer, F; Leitinger, G; Devetak, D.
The evidence on the degradation processes in the midgut epithelial cells of the larval antlion Euroleon nostras (Geoffroy in Fourcroy, 1785) (Myrmeleontidae, Neuroptera).
Micron. 2012; 43(5):651-665
Doi: 10.1016/j.micron.2011.11.012
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Leitinger Gerd
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- Abstract:
- We analysed structural differences between midgut epithelial cells of fed instar antlions' larvae Euroleon nostras and starved ones. In starved larvae the presence of autophagolysosome-like structures was observed, which are characteristic structures associated with autophagy. The results presented here provide insight supporting the role of autophagy as a cell survival mechanism for the periods of food deprivation. Additional structural changes in the cytoplasm were seen in the spherites. The ultrastructure and chemical composition of spherites in the midgut epithelial cells of first, second and third instar larvae were observed with light microscopy and transmission electron microscopy (TEM). A detailed characterization of the elemental composition of the spherites was studied using analytical electron microscopy; a combination of energy dispersive X-ray spectroscopy (EDXS), electron energy-loss spectroscopy (EELS) and energy filtering TEM (EFTEM) was applied. The structure and elemental composition of the spherites changed during the period of larval life. Moreover, changes in chemical composition were found between spherites from fed and starved E. nostras. In fed first instar larvae, the spherites contained an organic matrix, composed of C, N and O. In this matrix, P, Cl, Ca and Fe were detected. In starved first instar larvae, only C, N and P were present. The spherites of fed second instar larvae were rich in organic and inorganic elements and were composed of C, N, O, Na, Mg, P, S, Cl, K, Ca, Mn, Fe and Zn. In starved second instar larvae, the chemical elements N, O, P, Ca and Fe were found. In fed third instar larvae, the spherites contained C, N, O, Na, Mg, P, Cl, K, Ca, Mn, Fe, Co and Zn. In starved third larvae, C, O, Si, Ca, and Fe were detected. Generally, the spherites are exploited in starved larvae. These results suggest that the elemental supply of spherites may provide crucial support for physiological processes during starvation periods amongst E. nostras instar larvae. In some cases in fed second and fed third instar larvae, spherites were seen in the lumen of the midgut. Such spherites could serve as reservoirs for nontoxic waste material that cannot be metabolized.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Autophagy - physiology
- Digestive System - chemistry Digestive System - metabolism Digestive System - ultrastructure
- Epithelial Cells - chemistry Epithelial Cells - physiology Epithelial Cells - ultrastructure
- Insects - anatomy and histology Insects - chemistry Insects - physiology
- Larva -
- Microscopy, Electron, Transmission -
- Spectrometry, X-Ray Emission -
- Spectroscopy, Electron Energy-Loss -
- Starvation - metabolism
- Find related publications in this database (Keywords)
- Autophagy
- Euroleon nostras
- Larval instars
- Midgut
- Spherites
- Ultrastructure