Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schlagenhauf, A; Kozma, N; Leschnik, B; Wagner, T; Muntean, W.
Thrombin receptor levels in platelet concentrates during storage and their impact on platelet functionality.
Transfusion. 2012; 52(6):1253-1259
Doi: 10.1111/j.1537-2995.2011.03475.x
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Muntean Eugen
- Schlagenhauf Axel
- Co-Autor*innen der Med Uni Graz
- Kozma Noemi
- Leschnik Bettina
- Wagner Thomas
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- Abstract:
- BACKGROUND: Quality control of platelet (PLT) concentrates is challenging, due to PLT lesions, which are difficult to detect with routine methods. The search for reliable PLT lesion biomarkers is focused on the role of PLTs in primary hemostasis. PLT transfusions also have a significant impact on secondary hemostasis. In this phase, responsiveness of PLTs to small amounts of thrombin is crucial. PAR1 and PAR4 are protease-activated receptors and are responsible for thrombin reactivity of human PLTs. This study should elucidate if levels of those two receptors are changing in PLT concentrates during storage and if those changes have an impact on PLT aggregation and support of thrombin generation. STUDY DESIGN AND METHODS: PLT concentrates from buffy coat preparations were stored in SSP+ solution for 9 days at 22 +/- 2 degrees C on a horizontal flatbed agitator, and samples were taken daily for analysis. PAR1 and PAR4 levels were evaluated using Western blot analysis. PLT aggregation was measured using Born aggregometry and specific PAR1 or PAR4 agonists. Thrombin generation was measured using calibrated automated thrombography. RESULTS: Levels of both receptors (PAR1 and PAR4) started to decrease after 5 days of storage. PAR1-mediated PLT aggregation remained constant, whereas PAR4-mediated PLT aggregation decreased with storage time. Rate of thrombin generation was accelerated after 5 days of storage. CONCLUSION: Decreasing levels of PARs in PLT concentrates after 5 days of storage influenced PAR4-mediated, but not PAR1-mediated, aggregation. Thrombin generation with senescent PLTs was increased, which may be attributed to other mechanisms promoting increased phosphatidylserine exposure.
- Find related publications in this database (using NLM MeSH Indexing)
- Blood Platelets - cytology Blood Platelets - metabolism Blood Platelets - physiology
- Blood Preservation - methods Blood Preservation - standards
- Cell Shape -
- Humans -
- Platelet Activation - physiology
- Platelet Aggregation - physiology
- Platelet Count -
- Plateletpheresis - standards
- Quality Control -
- Receptor, PAR-1 - analysis Receptor, PAR-1 - metabolism
- Receptors, Thrombin - analysis Receptors, Thrombin - metabolism Receptors, Thrombin - physiology
- Time Factors -