Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Harbaum, L; Pollheimer, MJ; Kornprat, P; Lindtner, RA; Schlemmer, A; Rehak, P; Langner, C.
Keratin 20 - a diagnostic and prognostic marker in colorectal cancer?
Histol Histopathol. 2012; 27(3):347-356
Web of Science
PubMed
- Leading authors Med Uni Graz
- Langner Cord
- Co-authors Med Uni Graz
- Kornprat Peter
- Pollheimer Marion
- Rehak Peter
- Schlemmer Andrea
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- Abstract:
- Colorectal cancer cells characteristically show strong expression of keratin 20 (K20) and lack expression of keratin 7 (K7). The biological significance of reduced K20 expression, however, is unclear. 381 colorectal cancers with 148 corresponding metastases were evaluated for K20 and K7 expression by immunohistochemistry using a tissue microarray technique. K20 immunoreactivity was assessed semiquantitatively as either negative, low (<50% of cancer cells) or high (≥50% of cancer cells). Progression-free and cancer-specific survivals were determined using the Kaplan-Meier method. Expression of K20 was observed in 348 out of 372 (94%) evaluable primary tumors, with 135 (36%) cases showing low K20 and 213 (57%) cases high K20 expression, while 24 (6%) tumors completely lacked K20 immunoreactivity. Reduced K20 expression (lack of staining or low expression) was significantly associated with poor differentiation, large tumor size and mismatch repair deficiency, but did not significantly affect patients' outcome. Immunoreactivity of K20 and K7 in metastatic tissues matched well with that of corresponding primary tumors, with high concordance for lymph node (p<0.001) and distant metastases (p<0.001), respectively. In conclusion, our data illustrate the value of keratin subtyping in carcinoma of unknown primary (CUP) syndrome: K20 expression is common in colorectal cancer and the K20 high / K7 negative immunoprofile represents the predominant phenotype. Reduced K20 expression may, however, lead to false-negative assessment of metastatic deposits if only small amounts of tissue are obtained (e.g. in needle biopsies), particularly in poorly differentiated cancers. Reduced expression of K20 may be used to select tumors for microsatellite instability testing.
- Find related publications in this database (using NLM MeSH Indexing)
- -
- Biomarkers, Tumor - analysis
- Carcinoma - chemistry Carcinoma - mortality Carcinoma - secondary Carcinoma - therapy
- Cell Differentiation -
- Chi-Square Distribution -
- Colorectal Neoplasms - chemistry Colorectal Neoplasms - mortality Colorectal Neoplasms - pathology Colorectal Neoplasms - therapy
- DNA Mismatch Repair -
- DNA Repair Enzymes - analysis
- Disease-Free Survival -
- Female -
- Humans -
- Immunohistochemistry -
- Kaplan-Meier Estimate -
- Keratin-20 - analysis
- Keratin-7 - analysis
- Male -
- Neoplasm Grading -
- Neoplasm Invasiveness -
- Neoplasm Staging -
- Predictive Value of Tests -
- Time Factors -
- Tissue Array Analysis -
- Treatment Outcome -
- Tumor Burden -
- Find related publications in this database (Keywords)
- Colorectal carcinoma
- Keratin 20
- Keratin 7
- Carcinoma of unknown primary
- Prognosis