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Selected Publication:
Wakabayashi, I; Mayer, B; Groschner, K.
Inhibitory effects of aclarubicin on nitric oxide production in aortic smooth muscle cells and macrophages.
Biochem Pharmacol. 2000; 59(6):719-726
Doi: 10.1016/S0006-2952(99)00358-5
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- Co-authors Med Uni Graz
- Groschner Klaus
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- Abstract:
- The effects of aclaruhicin (ACR), an anthracycline antibiotic, on inducible nitric oxide (NO) synthesis was investigated in rat aortic smooth muscle cells (RASMCs) and RAW macrophages. ACR at concentrations as low as 0.1 microM significantly inhibited NO production induced by interleukin-1beta in RASMCs. About 5- to 10-fold higher concentrations of ACR were required for inhibition of interferon-gamma and lipopolipopolysaccharide-induced NO production in RAW cells. When ACR was subsequently administered to inducible NO synthase (iNOS) induction, the NO production was barely suppressed in RASMCs. Moreover, ACR (up to 10 microM) lacked direct inhibitory effects on iNOS activity in homogenates of these cells. ACR (0.1 microM) inhibited the expression of iNOS protein and mRNA in RASMCs without concomitant cytotoxic effects. ACR (>0.5 microM)-induced inhibition of NO production in RAW cells was associated with substantial cytotoxic effects as shown by measurement of lactate dehydrogenase release. These results suggest that ACR is a potent inhibitor of iNOS induction in vascular smooth muscle, but inhibits iNOS induction in macrophage only at high cytotoxic
- Find related publications in this database (using NLM MeSH Indexing)
- Aclarubicin - pharmacology
- Animals -
- Antibiotics, Antineoplastic - pharmacology
- Aorta - cytology
- Cell Division - drug effects
- Cells, Cultured -
- Dichlororibofuranosylbenzimidazole - pharmacology
- Enzyme Inhibitors - pharmacology
- Macrophages - drug effects Macrophages - metabolism
- Male -
- Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism
- Nitric Oxide - metabolism
- Nitric Oxide Synthase - biosynthesis Nitric Oxide Synthase - metabolism
- Nitric Oxide Synthase Type II -
- RNA, Messenger - metabolism
- Rats -
- Rats, Wistar -
- Find related publications in this database (Keywords)
- aclarubicin
- vascular smooth muscle cell
- macrophage
- inducible nitric oxide synthase
- gene transcription
- cytotoxicity