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SHR Neuro Cancer Cardio Lipid Metab Microb

Liu, X; Groschner, K; Ambudkar, IS.
Distinct Ca(2+)-permeable cation currents are activated by internal Ca(2+)-store depletion in RBL-2H3 cells and human salivary gland cells, HSG and HSY.
J Membr Biol. 2004; 200(2):93-104 Doi: 10.1007/s00232-004-0698-3
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Co-authors Med Uni Graz: Groschner Klaus
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Abstract:: Store-operated Ca(2+) influx, suggested to be mediated via store-operated cation channel (SOC), is present in all cells. The molecular basis of SOC, and possible heterogeneity of these channels, are still a matter of controversy. Here we have compared the properties of SOC currents ( I(SOC)) in human submandibular glands cells (HSG) and human parotid gland cells (HSY) with I(CRAC) (Ca(2+) release-activated Ca(2+) current) in RBL cells. Internal Ca(2+) store-depletion with IP(3) or thapsigargin activated cation channels in all three cell types. 1 muM Gd(3+) blocked channel activity in all cells. Washout of Gd(3+) induced partial recovery in HSY and HSG but not RBL cells. 2-APB reversibly inhibited the channels in all cells. I(CRAC )in RBL cells displayed strong inward rectification with E(rev)(Ca) = >+90 mV and E(rev) (Na) = +60 mV. I(SOC) in HSG cells showed weaker rectification with E(rev)(Ca) = +25 mV and E(rev)(Na) = +10 mV. HSY cells displayed a linear current with E(rev) = +5 mV, which was similar in Ca(2+)- or Na(+)-containing medium. pCa/ pNa was >500, 40, and 4.6 while pCs / pNa was 0.1,1, and 1.3, for RBL, HSG, and HSY cells, respectively. Evidence for anomalous mole fraction behavior of Ca(2+)/Na(+) permeation was obtained with RBL and HSG cells but not HSY cells. Additionally, channel inactivation with Ca(2+) + Na(+) or Na(+) in the bath was different in the three cell types. In aggregate, these data demonstrate that distinct store-dependent cation currents are stimulated in RBL, HSG, and HSY cells. Importantly, these data suggest a molecular heterogeneity, and possibly cell-specific differences in the function, of these channels.
Find related publications in this database (using NLM MeSH Indexing): Cadmium - pharmacology; Calcium - metabolism; Calcium Channels - metabolism; Calcium Signaling - drug effects Calcium Signaling - physiology; Cations - metabolism; Cell Line -; Enzyme Inhibitors - pharmacology; Humans -; Inositol 1,4,5-Trisphosphate - pharmacology; Ion Channel Gating - drug effects; Membrane Potentials - drug effects; Parotid Gland - cytology Parotid Gland - physiology; Patch-Clamp Techniques -; Sarcoplasmic Reticulum - metabolism; Submandibular Gland - cytology Submandibular Gland - physiology; Thapsigargin - pharmacology
Find related publications in this database (Keywords): store-operated channels; calcium; cations; salivary epithelial cells; thapsigargin; IP