Medizinische Universität Graz - Research portal
Selected Publication:
Marumo, M; Suehiro, A; Kakishita, E; Groschner, K; Wakabayashi, I.
Extracellular pH affects platelet aggregation associated with modulation of store-operated Ca(2+) entry.
Thromb Res. 2001; 104(5):353-360
Doi: 10.1016/S0049-3848(01)00374-7
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-authors Med Uni Graz
- Groschner Klaus
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The pH dependence of store-operated Ca(2+) influx (SOCI) into human platelets, as well as its physiological consequence, aggregation, was studied. In Ca(2+)-free medium, thapsigargin (1 microM) induced a small increase in intracellular free-Ca(2+) ([Ca(2+)](i)), which was not affected by changes in extracellular pH. The addition of Ca(2+) (0.5-3 mM) after Ca(2+) store depletion caused by thapsigargin resulted in concentration-dependent increases in [Ca(2+)](i) (SOCI), which were strongly inhibited by SKF-96365 (100 microM), an inhibitor of receptor-mediated Ca(2+) entry. SOCI was inhibited by acidosis (pH 6.9) and augmented by alkalosis (pH 7.9). The addition of Ca(2+) (0.5-3 mM) to platelets, which were kept in Ca(2+)-free medium, slightly but significantly increased [Ca(2+)](i). This Ca(2+) leak entry was also decreased and increased by extracellular acidosis (pH 6.9) and alkalosis (pH 7.9), respectively, but not affected by SKF-96365. Neither thapsigargin (1 microM) stimulation in Ca(2+)-free solution nor elevation of extracellular Ca(2+) alone was sufficient to induce platelet aggregation. In contrast, the addition of Ca(2+) (1 mM) to platelets activated by thapsigargin resulted in aggregation, which was markedly inhibited by SKF-96365 (100 microM). Platelet aggregation associated with SOCI was also inhibited by extracellular acidosis (pH 6.9) and augmented by extracellular alkalosis (pH 7.9). These results suggest that acidosis-induced inhibition, as well as alkalosis-induced promotion of platelet aggregation, involve pH effects on SOCI.
- Find related publications in this database (using NLM MeSH Indexing)
- Blood Platelets - physiology
- Calcium - physiology
- Calcium Channel Blockers - pharmacology
- Enzyme Inhibitors - pharmacology
- Humans -
- Hydrogen-Ion Concentration -
- Imidazoles - pharmacology
- Ion Transport - drug effects
- Platelet Aggregation - drug effects
- Thapsigargin - pharmacology
- Find related publications in this database (Keywords)
- acidosis
- alkalosis
- Ca2+ store
- thapsigargin
- platelet aggregation
- Ca2+ channels