Medizinische Universität Graz - Research portal
Selected Publication:
Schuhmann, K; Groschner, K.
Protein kinase-C mediates dual modulation of L-type Ca2+ channels in human vascular smooth muscle.
FEBS Lett. 1994; 341(2-3):208-212
Doi: 10.1016/0014-5793(94)80458-3
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- Leading authors Med Uni Graz
- Groschner Klaus
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- Abstract:
- The role of protein kinase C (PKC) in cellular regulation of L-type Ca2+ channels was investigated in human umbilical vein smooth muscle. Activation of PKC, by low concentrations (< 30 nM) of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) caused inhibition of Ca2+ channels, while higher concentrations of TPA (> 100 nM) elicited a transient rise, followed by sustained inhibition of Ca2+ channel activity in cell-attached patches. Low TPA concentrations predominantly reduced channel availability, while high concentrations of TPA (100 nM) transiently increased channel availability and, in addition, prolonged mean open time. The inactive 4-alpha-phorbol-12,13- didecanoate failed to affect channel activity, and pretreatment of the cells with PKC inhibitors (H-7, chelerythrine) antagonized inhibitory and stimulatory effects of TPA. Our results provide evidence for two distinct PKC-dependent mechanisms of L-type Ca2+ channel regulation in smooth muscle.
- Find related publications in this database (using NLM MeSH Indexing)
- Calcium Channels - drug effects Calcium Channels - metabolism
- Humans -
- Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - enzymology Muscle, Smooth, Vascular - metabolism
- Protein Kinase C - metabolism
- Tetradecanoylphorbol Acetate - pharmacology
- Find related publications in this database (Keywords)
- L-TYPE CALCIUM CHANNEL
- CELLULAR REGULATION
- PHORBOL ESTER
- PROTEIN KINASE-C