Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

Brunner, F; Kühberger, E; Groschner, K; Pöch, G; Kukovetz, WR.
Characterization of muscarinic receptors mediating endothelium-dependent relaxation of bovine coronary artery.
Eur J Pharmacol. 1991; 200(1):25-33 Doi: 10.1016/0014-2999(91)90661-9
Web of Science PubMed FullText FullText_MUG Google Scholar

Co-authors Med Uni Graz: Groschner Klaus
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: In order to identify the receptor subtype responsible for acetylcholine (ACh)-induced relaxation of bovine coronary artery, we determined the affinity of six subtype-selective muscarinic antagonists and compared them with affinity estimates obtained for bovine left atria. At low concentrations, ACh potently relaxed circular strips of coronary artery with endothelium (EC50 0.15 microM), but contracted them at higher agonist concentrations with potencies that depended on the presence or absence of endothelium: EC50 1.8 microM (without endothelium); 4.6 microM (with endothelium). The pA2 values obtained for antagonism of relaxant responses to ACh were: pirenzepine (M1-selective) 7.38 +/- 0.12; AF-DX 116 (11-[2-(diethylamino-methyl)-1-piperidinyl-acetyl]-5,11- dihydro-6H-pyrido(2,3-b)1,4-benzodiazepine-6-one; M2-selective) 5.79 +/- 0.09; and 4-diphenylacetoxy-N-methyl-piperidine-methobromide (4-DAMP; M3/M1-selective) 9.07 +/- 0.12. The corresponding Schild slopes were 0.98 +/- 0.07 for pirenzepine, 1.17 +/- 0.09 for AF-DX 116 and 1.01 +/- 0.04 for 4-DAMP. For the following three antagonists, pKB values were determined at two different antagonist concentrations: dicyclomine (M1-selective) 7.49 +/- 0.10, cyclohexylphenyl-(2-piperidinoethyl)-silanol (CPPS; M3-selective) 8.0 +/- 0.10, and parafluoro-hexahydrosila-difenidol (pFHHSiD; M3-selective) 7.87 +/- 0.10. For comparison, the antagonism of methacholine-induced negative inotropy in left atria was determined for three antagonists, yielding the following pA2 values: pirenzepine 5.98 +/- 0.14; AF-DX 116 6.81 +/- 0.14 and 4-DAMP 7.99 +/- 0.14. The slopes of the corresponding Schild plots were 1.05 +/- 0.10, 1.14 +/- 0.12 and 1.08 +/- 0.08, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Find related publications in this database (using NLM MeSH Indexing): Acetylcholine - pharmacology; Animals -; Cattle -; Coronary Vessels -; Endothelium, Vascular - drug effects Endothelium, Vascular - physiology; Female -; Heart Atria - drug effects; Male -; Muscarinic Antagonists -; Muscle Contraction - drug effects; Muscle Relaxation - drug effects; Muscle, Smooth, Vascular - physiology; Myocardial Contraction - drug effects; Parasympatholytics - pharmacology; Vasodilation - drug effects
Find related publications in this database (Keywords): MUSCARINIC RECEPTORS; VASCULAR ENDOTHELIUM; CORONARY ARTERY (BOVINE); CHOLINERGIC VASODILATATION; (FUNCTIONAL EXPERIMENTS INVITRO)