Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Smolle, J; Helige, C; Soyer, HP; Hoedl, S; Popper, H; Stettner, H; Kerl, H; Tritthart, HA; Kresbach, H.
Quantitative evaluation of melanoma cell invasion in three-dimensional confrontation cultures in vitro using automated image analysis.
J INVEST DERMATOL 1990 94: 114-119.
Doi: 10.1111/1523-1747.ep12873976
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- Führende Autor*innen der Med Uni Graz
- Smolle Josef
- Co-Autor*innen der Med Uni Graz
- Helige Christine
- Hoedl Stefan
- Kerl Helmut
- Popper Helmuth
- Soyer Hans Peter
- Tritthart Helmut
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- Abstract:
- Tumor invasion is a crucial feature of tumor growth in vivo. Confrontation cultures of multicellular melanoma spheroids and embryonic chick heart fragments provide a model for invasive growth in vitro. We have developed an image analysis method, which facilitates the objective measurement of tumor cell invasion in this model. Cryostat sections of confrontation cultures were immunohistochemically stained with an antiserum directed against the stromal component for automated recognition of the stroma tissue. The slides were automatically processed by a grey level based computerized image analysis system. On Spearman's rank correlation test, 25 out of 39 parameters correlated with the reference value of invasion, which was derived from the subjective evaluation of five independent observers. Two parameters combining the stroma margin and the total amount of stroma tissue completely reproduced the judgement of the morphologists in our test set. The quantitative evaluation of tumor invasion in vitro by automated image analysis may be helpful in pharmacologic and pathogenetic studies of tumor growth.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Chick Embryo -
- Histological Techniques -
- Image Processing, Computer-Assisted -
- Melanoma - pathology
- Mice - pathology
- Myocardium - pathology
- Neoplasm Invasiveness - pathology
- Research Support, Non-U.S. Gov't - pathology
- Tumor Cells, Cultured - pathology