Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Jeanquartier, C; Schider, G; Feichtenhofer, S; Schwab, H; Schennach, R; Stettner, J; Winkler, A; Gruber-Woelfler, H; Schitter, G; Eder, RJ; Khinast, JG.
A two-step method to covalently bind biomolecules to group-IV semiconductors: Si(111)/1,2-epoxy-9-decene/esterase.
Langmuir. 2008; 24(24):13957-13961
Doi: 10.1021/la802075t
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- Führende Autor*innen der Med Uni Graz
- Jean-Quartier Claire
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- Abstract:
- A versatile two-step method has been developed that allows linking of biomolecules covalently to hydrogen-terminated group-IV semiconductors by means of epoxy-alkenes. First, the terminal C==C double bond of the alkene forms a covalent bond with the silicon, germanium, or diamond surface by UV-mediated hydrosilylation. The terminal oxirane moiety then reacts with the biomolecule. As a model system, we investigated the attachment of an esterase B to a Si(111) surface by means of the linker molecule 1,2-epoxy-9-decene. Samples were characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. The immobilized enzyme retained its activity and exhibited good long-term stability.
- Find related publications in this database (using NLM MeSH Indexing)
- Epoxy Compounds - chemistry
- Esterases - chemistry Esterases - metabolism
- Models, Molecular -
- Semiconductors -
- Silicon - chemistry
- Spectrum Analysis -