Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Fischerauer, E; Heidari, N; Neumayer, B; Deutsch, A; Weinberg, AM.
The spatial and temporal expression of VEGF and its receptors 1 and 2 in post-traumatic bone bridge formation of the growth plate.
J Mol Histol. 2011; 42(6): 513-522.
Doi: 10.1007/s10735-011-9359-x
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Amerstorfer Eva
- Co-Autor*innen der Med Uni Graz
- Deutsch Alexander
- Weinberg Annelie-Martina
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- Abstract:
- Injuries to growth plates may initiate the formation of reversible or irreversible bone-bridges, may leading to bone length discrepancy or axis deviation. As vascular invasion is essential for the formation of bone tissue, the aim of our study was to investigate the kinetic expression of Vascular Endothelial Growth Factor (VEGF) and its receptors R1 and R2 and the ingrowth of vessels in the formation of bone bridges in a rat physeal injury model. Quantitative Real-Time Polymerase Chain Reaction was performed for VEGF and its receptors. Samples from the proximal physis of the tibial bone were immunohistochemically evaluated for the expression of VEGF and its R1 and R2 receptors and Laminin. Morphologically, physeal bone bridge formation was validated by means of Magnetic Resonance Imaging. Kinetic expression of VEGF and VEGF-R1 mRNA documented a tendency towards an increase in expression on day 7. Histological analyses showed a hematoma containing bone debris on day 1 which was replaced with bony trabeculae by day 14, forming a bone bridge by day 28 which was preceded and accompanied by angiogenesis and consistent with MRI data. VEGF and VEGF-R2 was expressed on the debris within the hematoma and bone trabeculae from days 1 to 28. VEGF-R1 expression was only noted until day 14. The findings of our study suggest that physeal bone bridge formation is in part triggered by VEGF expression and associated with angiogenesis, which was shown to precede bone bridge formation and may be further stimulated through VEGF-positive bone debris.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Growth Plate - blood supply
- Hematoma - physiopathology
- Kinetics -
- Magnetic Resonance Imaging - methods
- Male -
- Osteogenesis - genetics
- Rats -
- Rats, Sprague-Dawley -
- Tibia - blood supply
- Vascular Endothelial Growth Factor A - biosynthesis
- Vascular Endothelial Growth Factor Receptor-1 - biosynthesis
- Vascular Endothelial Growth Factor Receptor-2 - biosynthesis
- Find related publications in this database (Keywords)
- Growth plate injury
- Bone bridge
- Vascular endothelial growth factor
- Laminin