Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Rinner, B; Froehlich, EV; Buerger, K; Knausz, H; Lohberger, B; Scheipl, S; Fischer, C; Leithner, A; Guelly, C; Trajanoski, S; Szuhai, K; Liegl, B.
Establishment and detailed functional and molecular genetic characterisation of a novel sacral chordoma cell line, MUG-Chor1.
Int J Oncol. 2012; 40(2): 443-451.
Doi: 10.3892/ijo.2011.1235
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- Leading authors Med Uni Graz
- Rinner Beate
- Co-authors Med Uni Graz
- Fischer Carina
- Fröhlich-Sorger Elke Verena
- Gülly Christian
- Knausz Heike
- Leithner Andreas
- Liegl-Atzwanger Bernadette
- Lohberger Birgit
- Scheipl Susanne
- Trajanoski Slave
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- Abstract:
- Chordomas are rare, low to intermediate grade malignant bone tumors of the axial skeleton. Current treatment options are limited to surgical procedures, as chordomas are largely resistant to conventional radiation and chemotherapy. Cell lines are valuable tools for exploring molecular mechan-isms involved in tumorigenesis and they have a fundamental impact on the development of new anticancer agents. To date, only two chordoma cell lines exist world-wide. In the present study we report a third chordoma cell line, MUG-Chor1, as well as corresponding cultured fibroblasts established from a recur-rent morphologically 'classic' sacrococcygeal chordoma of a 58-year-old Caucasian female. The cells are brachyury-positive and have the characteristics of chordoma. The genetic profile of the primary chordoma and the established chordoma cell line was investigated during the culturing period (early and late passage). MUG-Chor1 is karyotypically, <2n>43-47,XX,del(3)(q1?)[11], +7,del(9)(p1?),der(9;15)(q10;q10),-10,+der(12)t(9;12)(p2?;q1?),der (12)t(12;19)(p;p)t(17;19)(q;q),-15,der(17;21)(q10;q10),der(20)t(10;20) (q25?26?;q11?12?),-21,-22[20]/idemx2[5] and displays known, chordoma-typical genetic changes, such as chromosomal gains at T/brachyury locus (6q27), losses at 9p24.3-p13.1 (includes the CDKN2a/CDKN2b locus), 10p15.3-q23.32 (includes the PTEN locus) and losses of 10q25.2 (includes the PDCD4 locus). MUG-Chor1 bears a marked resemblance to chordomas in vivo and is, therefore, an optimal in vitro chordoma model.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line, Tumor - enzymology
- Chordoma - genetics
- Coccyx - pathology
- Female -
- Gene Dosage -
- Genotype -
- Humans -
- Karyotype -
- Loss of Heterozygosity -
- Middle Aged -
- Oligonucleotide Array Sequence Analysis -
- Polymorphism, Single Nucleotide -
- Sacrum - pathology
- Spinal Neoplasms - genetics
- Tumor Markers, Biological - genetics
- Find related publications in this database (Keywords)
- chordoma
- cell line
- brachyury
- matrix metalloproteinases