Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Flemming, A; Schallmoser, K; Strunk, D; Stolk, M; Volk, HD; Seifert, M.
Immunomodulative efficacy of bone marrow-derived mesenchymal stem cells cultured in human platelet lysate.
J Clin Immunol. 2011; 31(6):1143-1156
Doi: 10.1007/s10875-011-9581-z
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Schallmoser Katharina
- Strunk Dirk
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- Abstract:
- Human mesenchymal stem cells (hMSCs) are considered to be a promising tool for novel cell-based therapies. Clinical applications in solid organ transplantation were hampered by the dependence on animal serum for hMSCs clinical scale expansion until substitution with human platelet lysate (HPL) became a promising alternative. Therefore we focused on a direct comparison of immunomodulatory properties of hMSCs cultured in HPL or fetal calf serum (FCS). Phenotypic characterization, detection of cytokine secretion and effects on alloantigen- and mitogen-induced lymphocyte proliferation as well as degranulation of cytomegalovirus-specific cytotoxic T cells were applied in potency assays. We demonstrated that HPL-cultured MSCs have comparable immunomodulatory capacities to their FCS-cultured counterparts. The observed immunomodulatory properties include a beneficial inhibitory effect on immune cell proliferation and an unaffected viral T cell immunity. Thus, culturing hMSCs in HPL generates an efficient and safe expansion combined with intriguing immunomodulatory properties making these cells an attractive cell therapeutic tool.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, Viral - immunology
- Blood Platelets - metabolism
- Bone Marrow - pathology
- Cattle -
- Cell Extracts - immunology
- Cell Proliferation -
- Cells, Cultured -
- Culture Media - metabolism
- Cytomegalovirus - immunology
- Cytotoxicity, Immunologic -
- Humans -
- Immunomodulation -
- Isoantigens - immunology
- Mesenchymal Stem Cell Transplantation -
- Mesenchymal Stem Cells - immunology
- Serum - immunology
- T-Lymphocytes, Cytotoxic - immunology
- Find related publications in this database (Keywords)
- Immunomodulation
- mesenchymal stem cell
- human platelet lysate
- cell therapy