Medizinische Universität Graz - Research portal
Selected Publication:
Berg, J; Stöcher, M; Bogner, S; Wölfl, S; Pichler, R; Stekel, H.
Inducible cyclooxygenase-2 gene expression in the human thyroid epithelial cell line Nthy-ori3-1.
Inflamm Res. 2000; 49(4):139-143
Doi: 10.1007/PL00000204
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Berg Jörg
- Co-authors Med Uni Graz
- Pichler Robert
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- Abstract:
- OBJECTIVE: To investigate whether the genes encoding Cyclooxygenase-1 and -2 are expressed in thyroid epithelial cells, in vitro. MATERIALS AND METHODS: COX-1/-2 gene expression was examined in the thyroid epithelial cell line Nthy-ori3-1 using semi-quantitative RT-PCR and Western blot analysis. ELISAs were employed to assess whole cell COX-enzyme activity, PGE2 and IL-6 formation. RESULTS: In response to IL-1beta and TNF-alpha combined cells of the thyroid epithelial cell line Nthy-ori3-1 secreted marked amounts of PGE2 in a time-dependent fashion. This is attributed to increased levels of COX-2 specific mRNA, increased amounts of COX-2 protein and COX enzyme activity in the absence of detectable COX-1 protein. The inhibition of the induced COX enzyme activity by the selective COX-2 inhibitor NS-398 demonstrated the presence of COX-2 pharmacologically. The expression of the COX-2 gene was also accompanied by a marked induction of IL-6 formation, a well described inflammatory response of thyroid epithelial cells. CONCLUSIONS: Our observation presents first evidence that COX-2 gene expression is inducible in thyroid epithelial cells, in vitro, upon stimulation with the pro-inflammatory cytokines IL-1beta and TNF-alpha. This finding may indicate that thyroid epithelial cells could play an inflammatory controlling role perhaps during auto-immune thyroid diseases.
- Find related publications in this database (using NLM MeSH Indexing)
- Blotting, Western -
- Cell Line -
- Cyclooxygenase 1 -
- Cyclooxygenase 2 -
- Dinoprostone - biosynthesis
- Enzyme Induction - drug effects
- Epithelial Cells - metabolism
- Humans -
- Interleukin-1 - biosynthesis
- Isoenzymes - biosynthesis Isoenzymes - genetics
- Membrane Proteins -
- Prostaglandin-Endoperoxide Synthases - biosynthesis Prostaglandin-Endoperoxide Synthases - genetics
- RNA, Messenger - biosynthesis
- Thyroid Gland - cytology Thyroid Gland - metabolism
- Tumor Necrosis Factor-alpha - biosynthesis
- Find related publications in this database (Keywords)
- COX-1/-2
- IL-1 beta
- IL-6
- TNF-alpha
- thyroid epithelial cells
- NS-398