Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Du, F; Wang, J; Gao, Y; Wang, H; Wang, Q; Jiang, S; Goswami, N.
A hind limb disuse model inducing extensor digitorum longus atrophy in rats: tail suspension-immobilization.
Aviat Space Environ Med. 2011; 82(7): 689-693.
Doi: 10.3357/ASEM.2984.2011
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Goswami Nandu
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- Abstract:
- Du F, WANG J, GAO Y, WANG H, WANG Q, JIANG S, GOSWAMI N. A hind limb disuse model inducing extensor digitorum longus atrophy in rats: tail suspension-immoblilization. Aviat Space Environ Med 2011; 82:689-93. Background: This study aimed to examine the effects of a new hind limb disuse model on the morphology and fiber type distribution of the extensor digitorum longus (EDL) of rats. Quicker onset of disuse atrophy would reduce the amount of time the animals have to be subjected to tail suspension. Methods: The new hind limb disuse mode was achieved by tail suspension (TS) and one hind limb fixed by plaster-casting (tail suspension-immobilization, TS+lm). There were 42 female Sprague-Dawley rats randomly divided into 7 groups (6 rats in each group) and exposed to the conditions for 0-6 wk, respectively. The cross-sectional area (CSA) and the types of muscle fiber of the rats' EDL were measured and classified by histochemical staining. Results: The decrease of EDL's CSA caused by TS+lm was more obvious (significant change was observed after 4 wk and Type I fiber's CSA decreased 27.3% after 6 wk) than that caused by TS only (significant change occurred after 6 wk and Type I fiber's CSA diminished 18.01% at that time). TS lead to the transition of Type IIB fibers to Type I and IIA fibers while TS+lm led to Type I and IIA fibers getting converted to Type IIB fibers. Discussion: When compared to TS only, TS+lm caused faster and greater reduction of the EDL's CSA in rats. TS+lm can also lead to a shift from oxidative to glycolytic metabolism of the EDL muscle fiber. Tail suspension-immobilization is a new hind limb disuse model for the EDL and may be more effective.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Female -
- Hindlimb Suspension - methods
- Models, Animal -
- Muscle Fibers, Fast-Twitch - pathology
- Muscle Fibers, Skeletal - pathology
- Muscle Fibers, Slow-Twitch - pathology
- Muscular Atrophy - pathology
- Rats -
- Rats, Sprague-Dawley -
- Find related publications in this database (Keywords)
- tail suspension
- immobilization
- disuse atrophy
- muscle fibre type
- skeletal muscle morphology change