Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Petry, KU; Schmidt, D; Scherbring, S; Luyten, A; Reinecke-Lüthge, A; Bergeron, C; Kommoss, F; Löning, T; Ordi, J; Regauer, S; Ridder, R.
Triaging Pap cytology negative, HPV positive cervical cancer screening results with p16/Ki-67 Dual-stained cytology.
Gynecol Oncol. 2011; 121(3):505-509
Doi: 10.1016/j.ygyno.2011.02.033
Web of Science
PubMed
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FullText_MUG
- Co-authors Med Uni Graz
- Regauer Sigrid
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- Abstract:
- Testing for human papillomavirus (HPV) has been shown to increase the sensitivity and negative predictive value for detection of high-grade cervical intraepithelial neoplasia (CIN2+), either when used in conjunction with Pap cytology testing or alone. However, there is no satisfying clinical management algorithm for women testing Pap negative/HPV positive. We therefore evaluated the clinical utility of a novel dual biomarker-based approach (p16/Ki-67 Dual-stained cytology) for the identification of CIN2+ in women with Pap negative/HPV positive screening results, without the need to refer all women to immediate colposcopy. All women aged ≥30 enrolled during 2007/2008 into a regional prospective Pap/HPV co-testing screening pilot project and tested Pap negative, but positive for HPV (n=425) were included in the analysis. p16/Ki-67 Dual-stained cytology was performed from residual cellular material available from the liquid-based cytology vial collected during the initial Pap/HPV co-testing screening visit. Results were correlated to the presence of CIN2+ confirmed during preliminary follow-up. p16/Ki-67 Dual-stained cytology tested positive at baseline in 108 out of 425 (25.4%) Pap negative/HPV positive cases. Sensitivity of Dual-stain testing for the detection of biopsy-confirmed CIN2+ during preliminary follow-up within the group of Pap negative/HPV positive women was 91.9% for CIN2+ (34/37 cases), and 96.4% for CIN3+ (27/28 cases). Specificity was 82.1% for CIN2+ on biopsy, and 76.9% for CIN3+, respectively. Triaging Pap negative/HPV positive screening test results with p16/Ki-67 Dual-stained cytology may identify women with a high probability of underlying CIN2+ and may efficiently complement HPV-based screening programs to prevent cervical cancer. Copyright © 2011 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Cervical Intraepithelial Neoplasia - metabolism
- Cervical Intraepithelial Neoplasia - pathology
- Cervical Intraepithelial Neoplasia - virology
- Cohort Studies -
- Cyclin-Dependent Kinase Inhibitor p16 -
- Female -
- Humans -
- Ki-67 Antigen - metabolism
- Middle Aged -
- Neoplasm Proteins - metabolism
- Papillomavirus Infections - metabolism
- Papillomavirus Infections - pathology
- Papillomavirus Infections - virology
- Sensitivity and Specificity -
- Staining and Labeling - methods
- Uterine Cervical Neoplasms - metabolism
- Uterine Cervical Neoplasms - pathology
- Uterine Cervical Neoplasms - virology
- Vaginal Smears -
- Find related publications in this database (Keywords)
- Pap cytology
- Human papillomavirus, HPV
- p16(INK4a)
- Ki-67
- Dual-stained cytology
- Cervical intraepithelial neoplasia