Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hasmann, A; Gewessler, U; Hulla, E; Schneider, KP; Binder, B; Francesko, A; Tzanov, T; Schintler, M; Van der Palen, J; Guebitz, GM; Wehrschuetz-Sigl, E.
Sensor materials for the detection of human neutrophil elastase and cathepsin G activity in wound fluid.
EXP DERMATOL. 2011; 20(6): 508-513.
Doi: 10.1111/j.1600-0625.2011.01256.x
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Binder Barbara
- Schintler Michael
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- Abstract:
- Human neutrophil elastase (HNE) and cathepsin G (CatG) are involved in the pathogenesis of a number of inflammatory disorders. These serine proteinases are released by neutrophils and monocytes in case of infection. Wound infection is a severe complication regarding wound healing causing diagnostic and therapeutic problems. In this study we have shown the potential of HNE and CatG to be used as markers for early detection of infection. Significant differences in HNE and CatG levels in infected and non-infected wound fluids were observed. Peptide substrates for these two enzymes were successfully immobilised on different surfaces, including collagen, modified collagen, polyamide polyesters and silica gel. HNE and CatG activities were monitored directly in wound fluid via hydrolysis of the chromogenic substrates. Infected wound fluids led to significant higher substrate hydrolysis compared with non-infected ones. These different approaches could be used for the development of devices which are able to detect elevated enzyme activities before manifestation of infection directly on bandages. This would allow a timely intervention by medical doctors thus preventing severe infections.
- Find related publications in this database (using NLM MeSH Indexing)
- Bandages -
- Biological Markers - analysis
- Cathepsin G - analysis
- Chromogenic Compounds -
- Exudates and Transudates - enzymology
- Humans -
- Leg Ulcer - diagnosis
- Leukocyte Elastase - analysis
- Pressure Ulcer - diagnosis
- Surgical Wound Infection - diagnosis
- Wound Healing - physiology
- Wound Infection - diagnosis
- Wounds and Injuries - enzymology
- Find related publications in this database (Keywords)
- cathepsin G
- diagnosis
- human neutrophil elastase
- immobilised substrates
- wound infection