Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kothmaier, H; Rohrer, D; Stacher, E; Quehenberger, F; Becker, KF; Popper, HH.
Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research.
Arch Pathol Lab Med. 2011; 135(6): 744-752.
Doi: 10.5858/2009-0676-OA.1
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Leading authors Med Uni Graz
- Kothmaier Hannelore
- Popper Helmuth
- Co-authors Med Uni Graz
- Quehenberger Franz
- Stacher-Priehse Elvira
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Context.-Formalin-fixed, paraffin-embedded tissue is the routine processing method for diagnostics practiced in pathology departments worldwide. Objective.-To determine the potential value of non-cross-linking, formalin-free tissue fixation for diagnostics in pathology and proteomic investigations. Design.-We tested 3 commercially available, formalin-free tissue fixatives-FineFIX, RCL2, and HOPE-in lung cancer specimens from 10 patients. The fixatives were evaluated for their effects on tissue morphology, protein recovery, and immunoreactivity for a selected panel of proteins differently expressed in lung cancer, using immunohistochemistry and Western blotting. Results.-Tumor-cell analysis with hematoxylin-eosin worked equally well for all tested fixatives when compared with the standard formalin-fixed, paraffin-embedded procedure. Movat pentachrome stains showed comparable results for the different matrices and cellular proteins analyzed. The RCL2 (P = .01) and HOPE fixatives (P = .03) improved protein recovery when compared with formalin-fixed, paraffin-embedded or frozen tissues. Our data clearly show that the fixatives evaluated influenced immunoreactivity to matched, formalin-fixed, paraffin-embedded lung cancer tissue. In particular, membrane-bound proteins, such as epidermal growth factor receptor EGFR, can be detected more efficiently by immunohistochemistry and Western blotting. Conclusion.-We have demonstrated that formalin-free fixatives have the potential in routine pathology and research to replace formalin in histomorphology and protein preservation. (Arch Pathol Lab Med. 2011;135:744-752)
- Find related publications in this database (using NLM MeSH Indexing)
- Blotting, Western -
- Fixatives -
- Formaldehyde -
- Frozen Sections -
- Humans -
- Immunohistochemistry - methods
- Lung Neoplasms - chemistry
- Neoplasm Proteins - analysis
- Paraffin Embedding -
- Pathology, Surgical - methods
- Proteomics - methods
- Receptor, Epidermal Growth Factor - analysis
- Tissue Fixation - methods
- Tumor Markers, Biological - analysis