Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Koidl, C; Riedl, R; Schweighofer, B; Fett, S; Bozic, M; Marth, E.
Performance of new enzyme-linked fluorescent assays for detection of Epstein-Barr virus specific antibodies in routine diagnostics.
Wien Klin Wochenschr. 2011; 123(7-8):230-234
Doi: 10.1007/s00508-011-1561-z
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Koidl Christoph
- Co-Autor*innen der Med Uni Graz
- Bozic Michael
- Fett Sandra
- Marth Egon
- Riedl Regina
- Schweighofer Bettina
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- Abstract:
- BACKGROUND AND AIMS: Indirect fluorescent antibody assays are still the gold standard for the detection of Epstein-Barr-virus-specific antibodies; however, this technique requires several manual steps and an experienced technician. This retrospective study investigated the performance of the new VIDAS(A (R)) enzyme-linked fluorescent assays for automated qualitative detection of EBV VCA IgM, VCA/EA IgG, and EBNA IgG antibodies in routine diagnostics. METHODS: 174 serum samples were tested first with the gold standard. In context with the clinical status, 60 samples IFA IgG/IgM positive and with clinical symptoms compatible with infectious mononucleosis, 26 samples IFA IgG/IgM negative and missing any clinical symptoms and 88 samples with varying IFA status and without any clinical information were defined. In a second step all samples were retested with the new assays. RESULTS: In the overall agreement between VIDAS(A (R)) and IFA for evaluable results, almost perfect agreement was observed (kappa = 0.91; 95% confidence interval (CI), 0.86-0.97). Estimating all indeterminate VIDAS(A (R)) results as discordant or concordant the observed kappa values were 0.71 (CI 0.63-0.79) and 0.93 (CI 0.88-0.98), respectively. With the new assays 45, 22, and 70 identical results were obtained, respectively. Western blot analysis of the discrepant samples showed a quasi similar performance of both assays. CONCLUSIONS: The new VIDAS(A (R)) assays can be an alternative to IFA testing especially in high-throughput laboratories. Full automation of EBV serological diagnostis by the new VIDAS assays is of major importance for routine diagnostic laboratories.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Viral - blood
- Diagnostic Tests, Routine - methods
- Enzyme-Linked Immunosorbent Assay - methods
- Epstein-Barr Virus Infections - blood
- Herpesvirus 4, Human - immunology
- Humans -
- Reproducibility of Results -
- Sensitivity and Specificity -
- Spectrometry, Fluorescence - methods
- Find related publications in this database (Keywords)
- EBV
- IFA
- ELFA
- automation
- Western blot