Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Werling, AM; Doerflinger, Y; Brandner, JM; Fuchs, F; Becker, JC; Schrama, D; Kurzen, H; Goerdt, S; Peitsch, WK.
Homo- and heterotypic cell-cell contacts in Merkel cells and Merkel cell carcinomas: heterogeneity and indications for cadherin switching.
Histopathology. 2011; 58(2):286-303
Doi: 10.1111/j.1365-2559.2011.03748.x
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Becker Jürgen Christian
- Schrama David
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- Abstract:
- Aims: Merkel cell carcinomas (MCCs) are rare but aggressive tumours associated recently with Merkel cell polyomavirus (MCV). As development and progression of several types of carcinomas can be promoted by changes in cell adhesion proteins, the aim of this study was to examine homo- and heterotypic cell contacts of Merkel cells and MCCs. Methods and results: Merkel cells of healthy glabrous epidermis and 52 MCCs were analysed by double-label immunostaining, immunofluorescence and confocal microscopy. Merkel cells were connected to keratinocytes by E- and P-cadherin, desmoglein 2 and desmocollin 2. In contrast, the vast majority of MCCs (90%) contained N-cadherin, but only 67% and 65% contained E- and P-cadherin, respectively. Interestingly, P-cadherin was absent significantly more frequently in lymph node metastases than in primary tumours and by trend in more advanced clinical stages. Moreover, major subsets of MCCs synthesized desmoglein 2 and, surprisingly, tight junction proteins. No significant differences were observed upon stratification for MCV DNA, detected in 84% of tumours by real-time polymerase chain reaction. Conclusions: Assuming that MCCs originate from Merkel cells, our data indicate a switch from E- and P-cadherin to N-cadherin during tumorigenesis. Whether the unexpected heterogeneity of junctional proteins can be exploited for prognostic and therapeutic purposes will need to be examined.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Aged, 80 and over -
- Antigens, Polyomavirus Transforming - isolation and purification
- Cadherins - metabolism
- Capsid Proteins - isolation and purification
- Carcinoma, Merkel Cell - metabolism
- Cell Adhesion - physiology
- Desmosomes - ultrastructure
- Female -
- Fluorescent Antibody Technique -
- Humans -
- Keratinocytes - metabolism
- Male -
- Merkel Cells - metabolism
- Microscopy, Confocal -
- Middle Aged -
- Polyomavirus Infections - complications
- Reverse Transcriptase Polymerase Chain Reaction -
- Skin Neoplasms - metabolism
- Find related publications in this database (Keywords)
- cadherin
- desmosome
- Merkel cell carcinoma
- Merkel cell virus
- tight junction