Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Skoch, A; Jírů, F; Dezortová, M; Krusinová, E; Kratochvílová, S; Pelikánová, T; Grodd, W; Hájek, M.
Intramyocellular lipid quantification from 1H long echo time spectra at 1.5 and 3 T by means of the LCModel technique.
J Magn Reson Imaging. 2006; 23(5):728-735
Doi: 10.1002/jmri.20574
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Svehlikova Eva
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- Abstract:
- Purpose: To introduce a method of independent determination of CH2 and CH3 components of intramyocellular lipids (IMCLs) by using long TE for spectra measurement and LCModel for spectra evaluation, to test this technique in controls and insulin- resistant subjects, and to compare results at 1.5 and 3 T. Materials and Methods: Eight healthy volunteers and 11 patients with type 2 diabetes mellitus under-went measurement using a 1.5-T MR scanner; six healthy volunteers were measured using a 3-T MR scanner. Spectra from the tibialis anterior muscle were acquired by using a point resolved spectroscopy (PRESS) sequence with the following parameters: TR/TE/ACQ = 2000 msec/270 msec/256. Spectra were processed by LCModel 6.1 software with two types of adopted basis-set. Results: Spectra with good separation of both CH2 and CH3 components of IMCL and extramyocellular lipids (EMCLs) were obtained and the LCModel routine was successfully applied. The reproducibility comparison (N = 7 at 1.5 T vs. N = 5 at 3T) showed that better results can be obtained at higher B-0 values. The comparison of the healthy and insulin-resistant subjects proved that both IMCL_CH2/Cr and IMCL_CH3/Cr ratios significantly differ. Conclusion: Long TE spectroscopy of the human muscle with IMCL quantification using the LCModel technique can detect changes in IMCL levels as well as help in the study of fatty acyl chain composition. Using a higher field strength increased the intra-individual reproducibility by approximately 150%.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Algorithms -
- Analysis of Variance -
- Diabetes Mellitus - metabolism
- Humans -
- Image Interpretation, Computer-Assisted -
- Lipid Metabolism - physiology
- Magnetic Resonance Spectroscopy - methods
- Magnetics -
- Middle Aged -
- Models, Biological -
- Muscle, Skeletal - metabolism
- Reference Values -
- Reproducibility of Results -
- Time Factors -
- Find related publications in this database (Keywords)
- H-1 MR spectroscopy
- intramyocellular lipids
- calf muscle
- LCModel
- insulin resistance