Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Tilz, GP; Wiltgen, M; Borkenstein, M.
On the occurrence of the diabetes-associated antigen GAD 65 in human sera.
Int Arch Allergy Immunol. 2011; 155(2):167-179
Doi: 10.1159/000319193
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Tilz Gernot
- Co-Autor*innen der Med Uni Graz
- Borkenstein Helmuth Martin
- Wiltgen Marco
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- Abstract:
- Background: Glutamic acid decarboxylase (GAD 65) is involved as an antigen in diabetes mellitus type 1 and is generally considered to be located intracellularly in pancreas beta-cells. In this study we demonstrate its appearance in 64 human sera samples representing a cross-section of a blood bank. Method: The proof of GAD 65 in sera was done using an enzyme-linked immunosorbent assay (ELISA) setup where it was detected by interaction with corresponding antibodies labeled with an enzyme. The enzyme catalyzes a substrate reaction, resulting in a change of color, that is used for the quantitative evaluation of the antigen-antibody interaction. Result: The measurements showed that GAD 65 exists in various amounts in the sera of blood donors, with an average concentration of 58.00 ng/ml. The correlation analysis of samples stored at -80 degrees C and at room temperature demonstrates the stability of GAD 65 at room temperature. The correlation coefficient between the GAD 65 concentrations of samples stored at room temperature and of the same samples after 1 week shows that the molecule remains stable. Conclusion: Our results encourage us to propose antigen GAD 65, due to its frequency in human sera in different concentrations and its stability, as a biomarker for the early diagnosis of type 1 diabetes and related inflammations. Copyright (c) 2010 S. Karger AG, Basel
- Find related publications in this database (using NLM MeSH Indexing)
- Autoantigens - blood
- Biological Markers - blood Biological Markers - chemistry
- Diabetes Mellitus, Type 1 - diagnosis Diabetes Mellitus, Type 1 - immunology
- Glutamate Decarboxylase - blood Glutamate Decarboxylase - chemistry
- Humans -
- Insulin-Secreting Cells - immunology Insulin-Secreting Cells - metabolism Insulin-Secreting Cells - pathology
- Protein Stability -
- Temperature -
- Find related publications in this database (Keywords)
- Glutamic acid decarboxylase (GAD 65)
- Diabetes mellitus type 1
- ELISA
- Biomarker