Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bondarenko, A; Chesler, M.
Calcium dependence of rapid astrocyte death induced by transient hypoxia, acidosis, and extracellular ion shifts.
Glia. 2001; 34(2):143-149
Doi: 10.1002/glia.1049
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Bondarenko Oleksandr
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- Abstract:
- Exposure to hypoxic, acidic, ion-shifted Ringer (HAIR) for 15-40 min has been shown to cause rapid astrocyte death upon reperfusion with normal media. The ion shifts of the HAIR solution included a rise in extracellular K(+) (e.g., [K(+)](o)) and a fall in [Na(+)](o), [Cl(-)](o), and [Ca(2+)](o), characteristic of ischemic-traumatic brain insults. We investigated the ionic basis of the HAIR-induced injury. After HAIR exposure, reperfusion in 0 Ca(2+)/EGTA media completely protected astrocytes. Preincubation of cells in BAPTA-AM ester was also protective, indicating that the injury was triggered by Ca(2+) influx during reperfusion. Neither nimodipine, CNQX, APV, nor TTX reduced injury. Astrocyte death could be blocked by 100 microM Ni(2+) or 100 microM benzamil, suggesting involvement of Na(+)-Ca(2+) exchange. KB-R7943, which preferentially inhibits reverse Na(+)-Ca(2+) exchange, also protected astrocytes. Elevation of [K(+)](o) was not necessary for astrocyte death. However, when [Na(+)](o) was maintained at 151 mM throughout the HAIR protocol, cell death was markedly reduced. We postulate that [Na(+)](o) shifts aid reversal of Na(+)-Ca(2+) exchange by favoring cytosolic Na(+) loading. Possible means of astrocytic Na(+) accumulation are discussed.
- Find related publications in this database (using NLM MeSH Indexing)
- Acidosis - metabolism
- Animals -
- Astrocytes - cytology Astrocytes - metabolism
- Biological Transport - drug effects Biological Transport - physiology
- Calcium - pharmacokinetics
- Cell Death - physiology
- Cell Hypoxia - physiology
- Cells, Cultured -
- Chelating Agents - pharmacology
- Chlorides - pharmacokinetics
- Egtazic Acid - analogs and derivatives Egtazic Acid - pharmacology
- Extracellular Space - metabolism
- Fluoresceins -
- Fluorescent Dyes -
- Ischemic Attack, Transient - metabolism
- Isotonic Solutions - chemistry
- Potassium - pharmacokinetics
- Rats -
- Sodium - pharmacokinetics
- Find related publications in this database (Keywords)
- ischemia
- BCECF
- BAPTA-AM
- Na+-Ca2(+) exchange
- benzamil
- KB-R7943