Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Rappl, G; Kapsokefalou, A; Heuser, C; Rössler, M; Ugurel, S; Tilgen, W; Reinhold, U; Abken, H.
Dermal fibroblasts sustain proliferation of activated T cells via membrane-bound interleukin-15 upon long-term stimulation with tumor necrosis factor-alpha.
J Invest Dermatol. 2001; 116(1):102-109
Doi: 10.1046/j.1523-1747.2001.00239.x
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-Autor*innen der Med Uni Graz
- Ugurel-Becker Selma
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- In chronic inflammatory conditions, mononuclear cells infiltrate the connective tissue attracted by fibroblast-secreted chemokines. The role of fibroblasts in sustaining the lymphocyte immune response upon cellular infiltration is so far unresolved. We here report that, upon prolonged stimulation with tumor necrosis factor-alpha, dermal fibroblasts enhance proliferation of activated T cells whereas unstimulated fibroblasts do not. T cell growth stimulation requires cell contact of tumor necrosis factor-alpha stimulated fibroblasts to T cells and is not due to soluble factors. Growth stimulation is substantially blocked by neutralizing antibodies to interleukin-15. Fluorescence-activated cell sorter analyses revealed that tumor necrosis factor alpha stimulated fibroblasts expose interleukin-15 in a membrane-bound form on the cell surface whereas nonstimulated fibroblasts and interferon-gamma treated fibroblasts do not. The amount of membrane interleukin-15 increases with the duration of tumor necrosis factor-alpha stimulation for at least 3 d. Unstimulated fibroblasts, however, accumulate interleukin-15 in the cytoplasm. No interleukin-15 could be detected in the culture supernatant. Immunohistochemical analyses confirmed membrane interleukin-15 on dermal fibroblasts in discoid lupus erythematosus skin lesions whereas no membrane interleukin-15 was found on the surface of fibroblasts in healthy skin. We conclude that dermal fibroblasts upon long-term tumor necrosis factor-alpha stimulation during chronic inflammation are involved via membrane-bound interleukin-15 in stimulating proliferation of accumulated, activated T cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies -
- Cell Division - physiology
- Cell Membrane - chemistry
- Fibroblasts - cytology
- Humans -
- Immunoenzyme Techniques -
- Interleukin-15 - genetics Interleukin-15 - immunology Interleukin-15 - pharmacology
- Lupus Erythematosus, Discoid - pathology
- Lymphocyte Activation - drug effects
- RNA, Messenger - metabolism
- Reverse Transcriptase Polymerase Chain Reaction -
- Skin - cytology
- T-Lymphocytes - immunology
- Time Factors -
- Tumor Necrosis Factor-alpha - pharmacology
- Find related publications in this database (Keywords)
- cell-to-cell interactions
- human fibroblasts
- inflammation