Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Rappl, G; Abken, H; Hasselmann, DO; Tilgen, W; Ugurel, S; Reinhold, U.
The CD7(-) subset of CD4(+) memory T cells is prone to accelerated apoptosis that is prevented by interleukin-15 (IL-15).
Cell Death Differ. 2001; 8(4):395-402
Doi: 10.1038/sj.cdd.4400825
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- Co-Autor*innen der Med Uni Graz
- Ugurel-Becker Selma
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- Abstract:
- The CD7(-) subset of CD4(+) T cells reflects a stable differentiation state of post-thymic helper T cells with CD45R0(+)CD45RA(-) 'memory' phenotype. Here we report that CD4(+)CD7(-) T cells are prone to increased spontaneous apoptosis in vitro compared to CD4(+)CD7(+) T cells. Spontaneous apoptosis is prevented by IL-15, but not by IL-2. Moreover, IL-15 increases Bcl-2 and decreases CD95/Fas expression of CD7(-), but not of CD7(+) T cells. Because IL-15 is physiologically not secreted but expressed in a membrane-bound form, we cocultured T cells with TNF-alpha stimulated fibroblasts that expose membrane IL-15. TNF-alpha stimulated fibroblasts rescue CD4(+)CD7(-) T cells from apoptosis whereas unstimulated fibroblasts do not. Rescue from apoptosis requires cell-cell contact and is abolished by addition of neutralizing antibodies to IL-15. We conclude that membrane IL-15 prevents accelerated apoptosis of CD4(+)CD7(-) T cells. This mechanism may contribute to accumulation of CD7(-) T cells in chronic inflammatory skin lesions.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD7 - analysis
- Antigens, CD95 - metabolism
- Apoptosis - drug effects
- CD4-Positive T-Lymphocytes - cytology CD4-Positive T-Lymphocytes - immunology
- Cells, Cultured -
- Dermatitis - immunology
- Dermis - cytology
- Fibroblasts - drug effects Fibroblasts - physiology
- Humans -
- Immunologic Memory -
- Immunophenotyping -
- Interleukin-15 - pharmacology
- Proto-Oncogene Proteins c-bcl-2 - metabolism
- Receptors, Interleukin-15 -
- Receptors, Interleukin-2 - metabolism
- T-Lymphocyte Subsets - classification T-Lymphocyte Subsets - cytology T-Lymphocyte Subsets - immunology
- Tumor Necrosis Factor-alpha - pharmacology
- Find related publications in this database (Keywords)
- membrane IL-15
- CD4(+)CD7(-) T cells
- memory cells
- fibroblasts