Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Rappl, G; Muche, JM; Abken, H; Sterry, W; Tilgen, W; Ugurel, S; Reinhold, U.
CD4(+)CD7(-) T cells compose the dominant T-cell clone in the peripheral blood of patients with Sézary syndrome.
J Am Acad Dermatol. 2001; 44(3):456-461
Doi: 10.1067/mjd.2001.110900
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Ugurel-Becker Selma
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Background: Absence of CD7 antigen expression in T cells defines a subset of normal CD4(+) CD45RO(+) CD45RA(-) memory cells and is furthermore observed in Sezary syndrome (SS). Objective: Our purpose was to identify circulating T-cell clones in patients with SS and to elucidate whether the dominant T-cell clones express the CD7 antigen. Methods: Peripheral blood lymphocytes of patients with SS were analyzed by two-color flow cytometry using antibodies to the V beta region of the T cell receptor (TCR) in combination with an antibody to CD7. In addition, T cells were analyzed for TCR-gamma gene rearrangement by polymerase chain reaction (PCR) techniques. Results: Clonal T-cell expansion was detected in 7 patients with SS by immunostaining of the TCR VP regions. PCR analysis confirmed the presence of dominant T cell clones. Double-immunostaining revealed that in each case cells of the clonal V beta TCR rearrangement homogeneously express the CD4(+)CD7(-) phenotype. Furthermore, CD4(+)CD7(-) cells express the CD15s antigen but lack expression of CD26 and CD49d. Conclusion: Expansion of clonal T cells strongly correlates with the expansion of CD4(+)CD7(-) T cells in 7 tested patients with SS. This supports our model that a subset of late differentiated, normal CD4(+)CD7(-) memory T cells may represent the physiologic counterpart of Sezary cells. Monitoring of circulating T cells with the CD4(+)CD7(-)CD15s(+)CD26(-)CD49d(-) phenotype proved to be useful for the identification of clonal T cells in patients with SS.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Antigens, CD4 - analysis
- Antigens, CD7 - analysis
- Cell Differentiation -
- Cell Division -
- Clone Cells -
- Female -
- Flow Cytometry -
- Genes, T-Cell Receptor gamma -
- Humans -
- Male -
- Middle Aged -
- Polymerase Chain Reaction -
- Sezary Syndrome - immunology
- T-Lymphocytes - immunology