Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
STOCKL, J; MAJDIC, O; ROSENKRANZ, A; FIEBIGER, E; KNIEP, B; STOCKINGER, H; KNAPP, W.
Monoclonal antibodies to the carbohydrate structure Lewis(x) stimulate the adhesive activity of leukocyte integrin CD11b/CD18 (CR3, Mac-1, alpha m beta 2) on human granulocytes.
J Leukoc Biol. 1993; 53(5):541-549
Doi: 10.1002/jlb.53.5.541
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Rosenkranz Alexander
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- Abstract:
- Several carbohydrate structures on human granulocytes have been discussed as potential ligands for C-type lectins (selectins) on endothelial cells. Among them are the lacto-series type II chain antigens sialyl-Lewis(x) (SLe(x), Lewis(x) (Le(x)), and VIM2. We demonstrated in this study that monoclonal antibodies (mAbs) to Le(x) and to SLe(x), but not other anticarbohydrate mAbs (VIM2, CDw17, CD24), can stimulate granulocytes to form homotypic aggregates. This effect was particularly noticeable with three distinct anti-Le(x) mAbs (3C6, 4D1, 6C7). Much less impressive effects were also seen with nine other anti-Le(x) mAbs and with the anti-SLe(x) mAb CSLEX1. Aggregation was shown to be an active process. It is temperature and energy dependent, requires divalent cations, and is selective in terms of mAb specificity. Anti-Le(x)-induced homoaggregate formation could be inhibited with CD11b mAb JML-H11 and CD54 (ICAM1) mAb LB-2 and thus seems to be associated with activation of the beta 2-integrin cytoadhesion pathway.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Monoclonal - analysis Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacology
- Antibody Specificity -
- Antigens, CD - analysis Antigens, CD - metabolism Antigens, CD - physiology
- Antigens, CD11 -
- Antigens, CD15 - immunology
- Antigens, CD18 -
- Cell Adhesion - drug effects Cell Adhesion - immunology
- Cell Adhesion Molecules - analysis Cell Adhesion Molecules - metabolism Cell Adhesion Molecules - physiology
- Enzyme-Linked Immunosorbent Assay -
- Fluorescent Antibody Technique -
- Glycolipids - analysis Glycolipids - immunology
- Glycoproteins - analysis Glycoproteins - immunology
- Granulocytes - cytology Granulocytes - immunology Granulocytes - physiology
- Humans -
- Immunoblotting -
- Intercellular Adhesion Molecule-1 -
- Leukocytes - cytology Leukocytes - immunology Leukocytes - physiology
- Macrophage-1 Antigen - analysis Macrophage-1 Antigen - metabolism Macrophage-1 Antigen - physiology
- Temperature -
- Find related publications in this database (Keywords)
- LECTIN-CARBOHYDRATE INTERACTIONS
- HOMOTYPIC AGGREGATION
- BETA(2)-INTEGRIN CYTOADHESION PATHWAY