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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Gauster, M; Berghold, VM; Moser, G; Orendi, K; Siwetz, M; Huppertz, B.
Fibulin-5 expression in the human placenta.
Histochem Cell Biol. 2011; 135(2): 203-213.
Doi: 10.1007/s00418-011-0784-4
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Gauster Martin
- Co-authors Med Uni Graz
- Aubell Kristina
- Berghold Veronika Marlies
- Huppertz Berthold
- Moser Gerit
- Siwetz Monika
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- Abstract:
- Fibulin-5 is a secreted extracellular matrix glycoprotein and displays a diverse panel of biological functions, which can be segregated into elastogenic as well as extra-elastogenic functions. While elastogenic functions of fibulin-5 include essential roles in early steps of elastic fibre assembly, extra-elastogenic functions are widespread. Depending on the cell type used, fibulin-5 mediates cell adherence via a subset of integrins, antagonizes angiogenesis and inhibits migration as well as proliferation of endothelial and smooth muscle cells. In this study, we focused on the spatiotemporal expression of fibulin-5 in the human placenta. With progressing gestation, placental fibulin-5 expression increased from first trimester towards term. At term, placental fibulin-5 mRNA expression is lower when compared with other well-vascularized organs such as lung, kidney, heart, uterus and testis. In first trimester, placenta immunohistochemistry localized fibulin-5 in villous cytotrophoblasts and extravillous cytotrophoblasts of the proximal cell column. In term placenta, fibulin-5 was detected in the endothelial basement membrane and adventitia-like regions of vessels in the chorionic plate and stem villi. Cell culture experiments with the villous trophoblast-derived cell line BeWo showed that fibulin-5 expression was downregulated during functional differentiation and intercellular fusion. Moreover, cultivation of BeWo cells under low oxygen conditions impaired intercellular fusion and upregulated fibulin-5 expression. The spatiotemporal shift from the trophoblast compartment in first trimester to the villous vasculature at term suggests a dual role of fibulin-5 in human placental development.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line -
- Extracellular Matrix Proteins - biosynthesis
- Female -
- Humans -
- Immunohistochemistry -
- Placenta - growth and development
- Pregnancy -
- Pregnancy Trimesters -
- RNA, Messenger - metabolism
- Find related publications in this database (Keywords)
- Human placenta
- Fibulin-5
- Trophoblast
- Extracellular matrix