Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Dingemann, J; Doi, T; Ruttenstock, E; Puri, P.
The role of primary myogenic regulatory factors in the developing diaphragmatic muscle in the nitrofen-induced diaphragmatic hernia.
Pediatr Surg Int. 2011; 27(6):579-582 Doi: 10.1007/s00383-010-2834-8
Web of Science PubMed FullText FullText_MUG

Co-authors Med Uni Graz: Ruttenstock Elke Maria
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: The nitrofen model of congenital diaphragmatic hernia (CDH) is widely used to investigate the pathogenesis of CDH. However, the exact pathomechanism of the diaphragmatic defect is still unclear. Diaphragmatic muscularization represents the last stage of diaphragmatic development. Myogenic differentiation 1 (MyoD) and myogenic factor 5 (Myf5) play a crucial role in muscularization. MyoD(-/-) : Myf5(+/-) mutant mice show reduced diaphragmatic size, whereas MyoD(+/-) : Myf5(-/-) mutants have normal diaphragms. We designed this study to investigate diaphragmatic gene expression of MyoD and Myf5 in the nitrofen CDH model. Pregnant rats received nitrofen or vehicle on day 9 of gestation (D9), followed by cesarean section on D18 and D21. Fetal diaphragms (n = 40) were micro-dissected and divided into CDH group and controls. MyoD and Myf5 mRNA-expression were determined using Real-time PCR. Immunohistochemistry was performed to evaluate protein expression of MyoD and Myf5. Relative diaphragmatic mRNA expression levels and immnunoreactivity of MyoD were decreased in the CDH group on D18 and D21. Myf 5 mRNA and protein expression were not altered in the CDH group. This is the first study showing that MyoD expression is selectively decreased in the diaphragm muscle in the nitrofen model of CDH.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Animals, Newborn -; Diaphragm - embryology; Disease Models, Animal -; Female -; Gene Expression Regulation, Developmental -; Hernia, Diaphragmatic - chemically induced; Immunohistochemistry -; MyoD Protein - biosynthesis; Myogenic Regulatory Factor 5 - biosynthesis; Phenyl Ethers - toxicity; Polymerase Chain Reaction -; Pregnancy -; Pregnancy, Animal -; RNA, Messenger - genetics; Rats -; Rats, Sprague-Dawley -
Find related publications in this database (Keywords): Congenital diaphragmatic hernia; Nitrofen; Diaphragmatic defect, MyoD, Myf5