Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schweintzger, S; Schlagenhauf, A; Rinner, B; Bernhard, H; Novak, M; Leschnik, B; Muntean, W.
High microparticle concentration in cord plasma.
HAMOSTASEOLOGIE. 2010; 30 Suppl 1(12): S122-S125.
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Muntean Eugen
- Schweintzger Sabrina
- Co-Autor*innen der Med Uni Graz
- Bernhard Heike
- Leschnik Bettina
- Novak Michael Peter
- Rinner Beate
- Schlagenhauf Axel
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- Abstract:
- We investigated if differences in the microparticle concentration and activity between newborn cord plasma and adult plasma exist. Methods: To enumerate and characterize microparticles (MP) FACS and ELISA were used.The effect of microparticles derived tissue factor (TF) on thrombin generation was measured indirectly by CAT (calibrated automated thrombography). Results: The flow cytometric measurements revealed an increased microparticle concentration in newborn cord compared with adult plasma. By the use of ELISA a significantly increased procoagulant activity of microparticles was found in newborn cord plasma as compared to adult plasma. Initiation of thrombin generation by adding phospholipids alone resulted in a significant lower prolongation of the lag time, time to peak in cord plasma, while the decrease of endogenous thrombin potential (ETP) and peak was comparable between newborns and adults. Conclusion: Our results show a higher impact of microparticles on the haemostatic system of newborns than on that of adults. The three methods suggest a somewhat increased microparticle activity in newborn cord plasma, but argue against strong platelet activation during birth.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Blood Chemical Analysis - methods
- Enzyme-Linked Immunosorbent Assay - methods
- Fetal Blood - chemistry Fetal Blood - cytology
- Flow Cytometry - methods
- Hemostasis -
- Humans -
- Infant, Newborn -
- Plasma - chemistry
- Platelet Activation -
- Find related publications in this database (Keywords)
- Microparticles
- FACS
- thrombin generation