Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kühn, KD; Weber, C; Kreis, S; Holzgrabe, U.
Evaluation of the stability of gentamicin in different antibiotic carriers using a validated MEKC method.
J Pharm Biomed Anal. 2008; 48(3):612-618
Doi: 10.1016/j.jpba.2008.05.041
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Kühn Klaus-Dieter
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- Abstract:
- The quality control of gentamicin in different antibiotic carriers, using MEKC as stability-indicating method is described. Baseline separations of gentamicin C1, C1a, C2, C2a and C2b and, furthermore the impurities and degradation products garamin (GARA), 2-deoxy-streptamine (DSA) and sisomicin (SISO) were achieved with a background electrolyte containing 20mM deoxycholic acid, 15 mM beta-cyclodextrin and 100mM tetraborate (pH 10.0). After derivatization with o-phthaldialdehyde reagent (OPA), UV detection at 340 nm was possible. The method was validated with respect to selectivity, limit of detection (LOD) and quantification (LOQ) of the impurities, linearity, accuracy, precision and robustness. Evaluation of four different antibiotic carriers stored under stability conditions according to the International Conference on Harmonization (ICH) guidelines and older pharmaceutical formulations disclosed good stability.
- Find related publications in this database (using NLM MeSH Indexing)
- Anti-Bacterial Agents - analysis Anti-Bacterial Agents - chemistry
- Borates - chemistry
- Chromatography, Micellar Electrokinetic Capillary - methods
- Deoxycholic Acid - chemistry
- Drug Delivery Systems -
- Drug Stability -
- Gentamicins - analysis Gentamicins - chemistry
- Hydrogen-Ion Concentration -
- Molecular Structure -
- Quality Control -
- Reproducibility of Results -
- Sensitivity and Specificity -
- beta-Cyclodextrins - chemistry
- Find related publications in this database (Keywords)
- Gentamicin
- MEKC
- Bone cement
- Long-term stability
- Quality control