Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Andersen, RS; Sørensen, RB; Ritter, C; Svane, IM; Becker, JC; thor Straten, P; Andersen, MH.
Identification of a cyclin B1-derived CTL epitope eliciting spontaneous responses in both cancer patients and healthy donors.
Cancer Immunol Immunother. 2011; 60(2):227-234
Doi: 10.1007/s00262-010-0933-y
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- Co-Autor*innen der Med Uni Graz
- Becker Jürgen Christian
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- Abstract:
- With the aim to identify cyclin B1-derived peptides with high affinity for HLA-A2, we used three in silico prediction algorithms to screen the protein sequence for possible HLA-A2 binders. One peptide scored highest in all three algorithms, and the high HLA-A2-binding affinity of this peptide was verified in an HLA stabilization assay. By stimulation with peptide-loaded dendritic cells a CTL clone was established, which was able to kill two breast cancer cell lines in an HLA-A2-dependent and peptide-specific manner, demonstrating presentation of the peptide on the surface of cancer cells. Furthermore, blood from cancer patients and healthy donors was screened for spontaneous T-cell reactivity against the peptide in IFN-γ ELISPOT assays. Patients with breast cancer, malignant melanoma, or renal cell carcinoma hosted powerful and high-frequency T-cell responses against the peptide. In addition, when blood from healthy donors was tested, similar responses were observed. Ultimately, serum from cancer patients and healthy donors was analyzed for anti-cyclin B1 antibodies. Humoral responses against cyclin B1 were frequently detected in both cancer patients and healthy donors. In conclusion, a high-affinity cyclin B1-derived HLA-A2-restricted CTL epitope was identified, which was presented on the cell surface of cancer cells, and elicited spontaneous T-cell responses in cancer patients and healthy donors.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Algorithms -
- Antibodies - blood
- Cells, Cultured -
- Cyclin B1 - chemistry
- Enzyme-Linked Immunosorbent Assay -
- Epitopes, T-Lymphocyte - immunology
- HLA-A2 Antigen - immunology
- Health -
- Humans -
- Middle Aged -
- Neoplasms - blood
- T-Lymphocytes, Cytotoxic - immunology
- Find related publications in this database (Keywords)
- Cyclin B1
- Tumor-associated antigen
- Cytotoxic T cells
- Anti-cancer vaccination
- CTL epitope