Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Cvirn, G; Tafeit, E; Hoerl, G; Janschitz, M; Wagner, T; Juergens, G; Koestenberger, M.
Heparinase-modified thrombelastometry: inactivation of heparin in plasma samples.
Clin Lab. 2010; 56(11-12): 585-589.
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Cvirn Gerhard
- Co-Autor*innen der Med Uni Graz
- Hörl Gerd
- Janschitz Michaela
- Jürgens Günther
- Koestenberger Martin
- Tafeit Erwin
- Wagner Thomas
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Abstract:
- Background: The heparinase-modified thrombelastometry (HEPTEM) assay is a promising tool to assess the coagulation status of heparinised patients. The aim of our study was to examine the heparin neutralizing capability of the HEPTEM assay in plasma samples. Methods: In the HEPTEM assay, blood or plasma samples become activated via the intrinsic pathway in the presence of a heparin processing enzyme. Results: We examined coagulation times (CTs) in the presence of increasing amounts (0 - 4 IU/mL) of heparin. We found that up to a concentration of 0.5 IU/mL, heparin is completely neutralized. However, CTs increased linearly in the presence of heparin concentrations higher than 0.5 IU/mL, indicating incomplete heparin neutralization in the standard HEPTEM assay. Conclusions: We provide herein a mathematical procedure to correct the misleadingly prolonged CTs (for heparin > 0.5 IU/mL) for the HEPTEM assay performed in plasma samples to allow better estimation of the coagulation status in patients requiring intense anticoagulation (e.g. patients undergoing cardiac surgery). (Clin. Lab. 2010;56:585-589)
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Blood Coagulation Tests - methods
- Drug Monitoring - methods
- Female -
- Heparin - blood
- Heparin Lyase - chemistry
- Humans -
- Linear Models -
- Male -
- Middle Aged -
- Thrombelastography - methods
- Thrombosis - blood
- Young Adult -
- Find related publications in this database (Keywords)
- Heparin
- heparinase
- thrombelastometry
- plasma
- neutralization